May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Expression of Organic Anion Transporters in the Ciliary Body Epithelium
Author Affiliations & Notes
  • R. M. Pelis
    Physiology, University of Arizona, Tucson, Arizona
  • M. Shahidullah
    Physiology, University of Arizona, Tucson, Arizona
  • M. Coca-Prados
    Department of Ophthalmology and Visual Sciences, Yale University, New Haven, Connecticut
  • N. A. Delamere
    Physiology, University of Arizona, Tucson, Arizona
  • Footnotes
    Commercial Relationships  R.M. Pelis, None; M. Shahidullah, None; M. Coca-Prados, None; N.A. Delamere, None.
  • Footnotes
    Support  NIH Grant EY006915-19
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3162. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      R. M. Pelis, M. Shahidullah, M. Coca-Prados, N. A. Delamere; Expression of Organic Anion Transporters in the Ciliary Body Epithelium. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3162. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : The interior of the eye is a pharmacological sanctuary to therapeutic drugs of clinical importance. Many of these drugs, including antivirals, antibiotics and chemotherapeutics are organic anions at physiological pH. Although it has been demonstrated that organic anions are cleared from the eye by a transport mediated process, the site(s) at which this occurs, and the transport proteins involved, are not well understood. Because the ciliary epithelium is a metabolically active tissue with a large transport capacity, it was hypothesized that organic anion transporters in the SLC22A, SLC13A and ABC families would be expressed in this tissue (with the expectation that they facilitate efflux of organic anions out of the eye).

Methods: : Reverse transcription polymerase chain reaction was used to look for mRNA expression of organic anion transporters in a human ciliary body cDNA library and cDNA isolated from primary cultures of porcine non-pigmented epithelium. Complementary DNA isolated from human or porcine kidney was used as positive controls. Sequencing was used to confirm amplification of the specific sequences.

Results: : mRNA corresponding to OAT1 (SLC22A6), MRP2 (ABCC2), MRP4 (ABCC4), and BCRP (ABCG2) were identified in the human ciliary body cDNA library. mRNA for the same transporters was found in primary cultures of porcine non-pigmented epithelium. MRP1 (ABCC1), NaDC1 (SLC13A2) and NaDC3 (SLC13A3) mRNA was not detected.

Conclusions: : These data indicate that several organic anion transporters common to other barrier and excretory tissues are expressed in the ciliary epithelium. The ciliary epithelium, and specifically these transporters, may influence drug efficacy in the eye.

Keywords: ciliary body • ion transporters • pump/barrier function 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×