May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Effect of Intracameral Injection of Gflx and Mflx on the Rabbit Corneal Endothelium
Author Affiliations & Notes
  • S. Kobayakawa
    Toho University, Tokyo, Japan
    Dept of Ophthalmology,
  • T. Arimura
    Toho University, Tokyo, Japan
    Dept of Ophthalmology,
  • T. Yamaguchi
    Ophthalmology, St.Luke's International Hospital, Tokyo, Japan
  • H. Watanabe
    Toho University, Tokyo, Japan
    1st Dept of Ophthalmology,
  • T. Tochikubo
    Toho University, Tokyo, Japan
    Dept of Ophthalmology,
  • Footnotes
    Commercial Relationships  S. Kobayakawa, None; T. Arimura, None; T. Yamaguchi, None; H. Watanabe, None; T. Tochikubo, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3176. doi:https://doi.org/
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    • Get Citation

      S. Kobayakawa, T. Arimura, T. Yamaguchi, H. Watanabe, T. Tochikubo; Effect of Intracameral Injection of Gflx and Mflx on the Rabbit Corneal Endothelium. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3176. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To determine the effect of intracameral injection of gatifloxacin (GFLX) 0.5% and moxifloxacin (MFLX) 0.5% on corneal endothelial cells of rabbits.

Methods: : Intracameral injection of GFLX 0.5% or MFLX 0.5% were performed at the end of cataract surgery with silicone IOL implantation on rabbit eyes. Injection volumes to each eye were 0.2ml. As a control, the saline was injected to those surgery eyes with silicone IOL implantation. Corneal central thickness of all rabbits was measured using by an ultrasonic pachymeter at one week post injection, and then those eyes were enucleated for observation of light microscopy (LM), and of transmission electron microscopy (TEM).

Results: : 1) Corneal central thickness for normal rabbit eyes were 434±22µm, thickness for control eyes (intracameral injection of saline) were 449±30µm, thickness for GFLX0.5% intracameral eyes were 648±42µm, thickness for MFLX0.5% intracameral eyes were 888±32µm. There was no difference for thickness between normal and control group. However, there were significant differences among intracameral antibiotics injection group, normal, and control group (p<0.01). Moreover, there was significant difference between GFLX0.5% intracameral group and MFLX0.5% group. 2) On TEM, corneal endothelial cells in intracameral antibiotics injection group showed slight organelle swelling, but did not show remarkable morphological changes.

Conclusions: : Postoperative corneal thickness increased significantly using by intracameral antibiotics injection, the values of intracameral MFLX0.5% group was the greatest among the groups. Those effects of intracameral antibiotics injection on the rabbit corneal endothelium were not remarkable.

Keywords: drug toxicity/drug effects • cornea: endothelium • endophthalmitis 
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