May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Characterization of Effector T cells in Dessicating Stress-Induced Keratoconjunctivits Sicca
Author Affiliations & Notes
  • J. El Annan
    Ophthalmology, Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, Massachusetts
  • S. K. Chauhan
    Ophthalmology, Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, Massachusetts
  • T. Ecoiffier
    Ophthalmology, Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, Massachusetts
  • D. R. Saban
    Ophthalmology, Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, Massachusetts
  • Q. Zhang
    Ophthalmology, Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, Massachusetts
  • R. Dana
    Ophthalmology, Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, Massachusetts
  • Footnotes
    Commercial Relationships  J. El Annan, None; S.K. Chauhan, None; T. Ecoiffier, None; D.R. Saban, None; Q. Zhang, None; R. Dana, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3235. doi:
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      J. El Annan, S. K. Chauhan, T. Ecoiffier, D. R. Saban, Q. Zhang, R. Dana; Characterization of Effector T cells in Dessicating Stress-Induced Keratoconjunctivits Sicca. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3235.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Dry eye syndrome (DES), one of the most common indications for patients seeking ophthalmic care, is associated with T cell recruitment to the ocular surface, suggesting that it is an immune-mediated disorder. The purpose of these experiments was to characterize the phenotype of the T cells activated in DES in a preclinical murine model of dry eye.

Methods: : Dry eye was induced in female C57BL/6 mice by exposure to a desiccating environment (relative humidity (RH) =22 ± 5%, airflow (AF) =15 L/min, temperature (T) = 21-23 oC) and to systemic scopolamine. Draining lymph nodes were harvested from dry eye and normal mice. The expression of T cell surface activation markers and chemokine receptors were analyzed by flow cytometry. T cell proliferation and cytokine secretion were detected by bromodeoxyuridine incorporation assay and ELISA respectively.

Results: : Draining lymph node (LN) T cells of dry eye mice expressed significantly higher levels of CD69 (p<0.05), and CCR5 (p<0.05). T cells from these LN showed a significant increase in IFN-γ (p<0.05) secretion, a decrease in IL-4 (p<0.05) secretion, and an increased proliferative capacity in response to CD3 stimulation.

Conclusions: : The increased expression of cell surface activation markers, proliferation, and higher secretion of IFN-γ, but not IL-4, indicates that a T helper-1 (Th1) type immune response is generated in regional LN in DES. These data provide further support for the hypothesis that DES is an autoimmune disease characterized by a regional or systemic Th1 response.

Keywords: cornea: tears/tear film/dry eye • inflammation • cornea: basic science 
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