Abstract
Purpose: :
To evaluate the protective effects of yellow tinted intraocular lenses (IOLs), investigate changes in visible light-induced photo-oxidization and the inhibitory effects of various acrylic tinted intraocular lenses (IOL) on photo-oxidation were analyzed experimentally.
Methods: :
Three types of non-tinted (VA-60BB, HOYA; SA60AT, Alcon; AU-6, Menicon) and tinted IOL (YA-60BB, HOYA; SN60AT, Alcon; AN-6, Menicon) were prepared. To investigate oxidation from visible light, cultured human retinal pigment epithelial cells were prepared, and cultured until confluent in a 96-well dish. The pigment epithelial cells were irradiated with artificial sunlight (Yamashita Denso) either directly or through various IOLs, for 30, 60, and 90 minutes. After light exposure lactate dehydrogenase (LDH) levels of the culture medium supernatant were measured to assess the amount of cell damage. Measurements of the amount of LDH precipitated as a result of cell damage was based on the detection of diformazan, the final product in the reaction system, at 560 nm. Statistical analysis was performed using a multiple comparison test (Fisher’s PLSD), with a significance level of p<0.05.
Results: :
LDH levels in retinal pigment epithelial cells increased to 1.2 mU/50 µl as a result of exposure to visible light. However, LDH level of non-tinted IOLs were 0.44 mU/50 µl (VA-60BB), 0.55 mU/50 µl (SA60AT), 0.63 mU/50 µl (AU-6) respectively. LDH level of tinted IOLs were 0.37 mU/50 µl (YA-60BB), 0.29 mU/50 µl (SN60AT), 0.30 mU/50 µl (AN-6) respectively. There are a statistically significant difference amoung control group, non-tinted IOL group and tinted IOL group.
Conclusions: :
Visible light causes photo-oxidation, which damages intraocular tissue. Results suggest that tinted IOLs are effective in inhibiting tissue damage from visible light.
Keywords: intraocular lens • clinical laboratory testing • stress response