May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Basic Fibroblast Growth Factor (bFGF) and Cytochrome C Peroxidase Association in Corneal Wound Healing in Excimer Laser Photo-Ablation
Author Affiliations & Notes
  • M. Morara
    Pathophysiological Optics, University of Bologna-Italy, Bologna, Italy
  • L. Scorolli
    Pathophysiological Optics, University of Bologna-Italy, Bologna, Italy
  • S. Z. Scalinci
    Pathophysiological Optics, University of Bologna-Italy, Bologna, Italy
  • A. Meduri
    Pathophysiological Optics, University of Bologna-Italy, Bologna, Italy
  • P. Ceruti
    University of Verona-Italy, Verona, Italy
  • P. Grenga
    University of Rome, Rome, Italy
  • G. Zigiotti
    Pathophysiological Optics, University of Bologna-Italy, Bologna, Italy
  • Footnotes
    Commercial Relationships  M. Morara, None; L. Scorolli, None; S.Z. Scalinci, None; A. Meduri, None; P. Ceruti, None; P. Grenga, None; G. Zigiotti, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3382. doi:
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      M. Morara, L. Scorolli, S. Z. Scalinci, A. Meduri, P. Ceruti, P. Grenga, G. Zigiotti; Basic Fibroblast Growth Factor (bFGF) and Cytochrome C Peroxidase Association in Corneal Wound Healing in Excimer Laser Photo-Ablation. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3382.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate the role of prepared Basic Fibroblast Growth Factor (bFGF) and cytochrome c peroxidase association eye-drops in corneal epithelial healing of mice ( B6(A)-Rperd12 /J ) after excimer laser photo-ablation.

Methods: : 240 eyes of 120 mice had photorefractive keratectomy (PRK). We divided four groups of 30 mice: A, B,C and D. In group A, both eyes received standard topical postoperative therapy cytochrome c peroxidase eyedrops 5ML 50000UI(3 times a day for 1 week or until corneal re-epithelialization was complete, corresponding to 15000UI/day). In group B, both eyes received standard topical postoperative therapy plus Basic Fibroblast Growth Factor eyedrops 5 µg / 10 µl PBS 3 times a day for 1 week. In group C, both eyes received standard topical postoperative therapy plus cytochrome c peroxidase eyedrops 5ML 50000UI (3 times a day corresponding to 15000 UI /day) and Basic Fibroblast Growth Factor eyedrops 5 µg / 10 µl PBS (3 times a day) for 1 week. In group D, both eyes received standard topical postoperative therapy. Mice were monitored daily starting the day after surgery and for 7 days to evaluate the corneal re-epithelialization rate using a video slit-lamp camera with a cobalt blue light. Mean diameter of corneal wounds was measured. Videotaped images were recorded and analyzed by computer planimetry.

Results: : All the eyes treated with cytochrome c peroxidase eyedrops healed completely before day 5 post-surgery, with a mean re-epithelialization time of 92 hours ± 10 (SD); all the eyes treated withBasic Fibroblast Growth Factor eyedrops healed completely before day 5 post-surgery, with a mean re-epithelialization time of 90 hours ± 12 (SD); all the eyes treated with association of cytochrome c peroxidase and Basic Fibroblast Growth Factor eyedrops healed completely before day 5 post-surgery, with a mean re-epithelialization time of 86 hours ± 12 (SD); the mean re-epithelialization time was 121 ± 8 hours in eyes receiving standard therapy (P<.05). No side effects or toxic effects were documented.

Conclusions: : This data suggests that cytochrome c peroxidase and bFGF association significantly accelerates epithelial healing after PTK.

Keywords: cornea: epithelium • growth factors/growth factor receptors • laser 
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