May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Delay in Epithelial Recovery After Corneal Refractive Surgery Increase Haze
Author Affiliations & Notes
  • S. del Olmo-Aguado
    University of Valladolid, Valladolid, Spain
    IOBA,
  • T. Blanco-Mezquita
    University of Valladolid, Valladolid, Spain
    IOBA,
  • P. Gallego-MuÑoz
    University of Valladolid, Valladolid, Spain
    IOBA,
  • L. Ibares-Frias
    University of Valladolid, Valladolid, Spain
    IOBA,
  • R. Cantalapiedra
    University of Valladolid, Valladolid, Spain
    IOBA,
  • A. Alvarez-Barcia
    University of Valladolid, Valladolid, Spain
    Animal facilities,
  • A. Mayo-Iscar
    University of Valladolid, Valladolid, Spain
    Statistics and O.R. Department,
  • J. Merayo-Lloves
    University of Valladolid, Valladolid, Spain
    IOBA,
  • Footnotes
    Commercial Relationships  S. del Olmo-Aguado, None; T. Blanco-Mezquita, None; P. Gallego-MuÑoz, None; L. Ibares-Frias, None; R. Cantalapiedra, None; A. Alvarez-Barcia, None; A. Mayo-Iscar, None; J. Merayo-Lloves, None.
  • Footnotes
    Support  FIS 052841
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3389. doi:https://doi.org/
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      S. del Olmo-Aguado, T. Blanco-Mezquita, P. Gallego-MuÑoz, L. Ibares-Frias, R. Cantalapiedra, A. Alvarez-Barcia, A. Mayo-Iscar, J. Merayo-Lloves; Delay in Epithelial Recovery After Corneal Refractive Surgery Increase Haze. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3389. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The aim of this work was to correlate the epithelium healing with the stromal healing in hens

Methods: : Thirty adult lohmann classic hens (60 eyes) were used. A mechanical deepithelization was made in a pre-marked area of 7 mm of diameter. PRK was performed in a 5.0-mm diameter optical zone and received -5.00 D of treatment (63 µm) using a Summit excimer laser. Animals were explored for epithelial closure, presence of haze and Histological changes. Epithelium closure was recorded with fluorescein dye under a surgical microscope with an incorporated digital camera at the following time points: 12, 24, 32, 40, 48 and 56 hours. Eyes were classified in four groups according the final time of epithelium closure: 32 hours (group 1), 40 hours (group 2), 48 hours (group 3) and 56 hours (groups 4).Haze was graded by method of Fantes (0-4; 0=clear cornea, 4 = Severely dense opacity) at 6, 15, 23 and 30 days after surgery.Corneas underwent pathology studies at 1 month and 3 months after surgery.Spearman correlation coefficient was assessed between day of epithelial recovery and haze. Descriptive study was performed for histology.

Results: : A positive correlation with statistical significant relation (p<0.05) was observed between the time of epithelium closure and grade of haze.New matrix production and increase in cell density in the anterior stroma was higher in group 4> group 3> group 2> group 1 one month after surgery.

Conclusions: : A rapid closure of epithelium integrity is necessary in order to decrease the grade of haze after corneal refractive surgery.

Keywords: antibiotics/antifungals/antiparasitics • cornea: stroma and keratocytes • drug toxicity/drug effects 
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