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S. del Olmo-Aguado, T. Blanco-Mezquita, P. Gallego-MuÑoz, L. Ibares-Frias, R. Cantalapiedra, A. Alvarez-Barcia, A. Mayo-Iscar, J. Merayo-Lloves; Delay in Epithelial Recovery After Corneal Refractive Surgery Increase Haze. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3389.
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The aim of this work was to correlate the epithelium healing with the stromal healing in hens
Thirty adult lohmann classic hens (60 eyes) were used. A mechanical deepithelization was made in a pre-marked area of 7 mm of diameter. PRK was performed in a 5.0-mm diameter optical zone and received -5.00 D of treatment (63 µm) using a Summit excimer laser. Animals were explored for epithelial closure, presence of haze and Histological changes. Epithelium closure was recorded with fluorescein dye under a surgical microscope with an incorporated digital camera at the following time points: 12, 24, 32, 40, 48 and 56 hours. Eyes were classified in four groups according the final time of epithelium closure: 32 hours (group 1), 40 hours (group 2), 48 hours (group 3) and 56 hours (groups 4).Haze was graded by method of Fantes (0-4; 0=clear cornea, 4 = Severely dense opacity) at 6, 15, 23 and 30 days after surgery.Corneas underwent pathology studies at 1 month and 3 months after surgery.Spearman correlation coefficient was assessed between day of epithelial recovery and haze. Descriptive study was performed for histology.
A positive correlation with statistical significant relation (p<0.05) was observed between the time of epithelium closure and grade of haze.New matrix production and increase in cell density in the anterior stroma was higher in group 4> group 3> group 2> group 1 one month after surgery.
A rapid closure of epithelium integrity is necessary in order to decrease the grade of haze after corneal refractive surgery.
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