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A. Inoue, Y. Okada, A. Kitano, O. Yamanaka, S. Saika; Effects of a Ppar-Gamma Ligand on Migration and Smad Activation in Corneal Epithelial Cell Line in vitro. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3400.
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© ARVO (1962-2015); The Authors (2016-present)
To determine whether or not troglitazone suppresses wound healing model in monolayer culture of the SV40- immortalized human corneal epithelial cell (HCEC) line. The perpxisome proliferation-activated receptor (PPAR) family consists of three members; PPAR-beta, -delta or -gamma which are involved in modulation of inflammatory cell function or cell proliferation during wound healing. Troglitazone is a ligand of PPAR-gamma.
Efects of Troglitazon on HCEC migration were examined subsequent to wounding confluent monolayer cultures. Alamar blue staining evaluated the effects of Troglitazone (0-10 microM) on HCEC proliferation. Immunostaining determined Smad3. One to 3 hours after wounding, Troglitazone inhibits nuclear translocation of Smad3 near the wound.
Troglitazon suppressed wound-induced cell migration and proliferation. Injury-induced Smad nuclear translocalization was also suppressed by the compound in HCEC monolayer culture.
Troglitazone suppresses wound healing in cultured HCEC sheet. These findings suggest that PPAR-gamma play a role in TGFb-driven corneal epithelial cell migration.
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