May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Effects of a Ppar-Gamma Ligand on Migration and Smad Activation in Corneal Epithelial Cell Line in vitro
Author Affiliations & Notes
  • A. Inoue
    Wakayama Medical University, Department of Ophthalmology, Wakayama, Japan
  • Y. Okada
    Wakayama Medical University, Department of Ophthalmology, Wakayama, Japan
  • A. Kitano
    Wakayama Medical University, Department of Ophthalmology, Wakayama, Japan
  • O. Yamanaka
    Wakayama Medical University, Department of Ophthalmology, Wakayama, Japan
  • S. Saika
    Wakayama Medical University, Department of Ophthalmology, Wakayama, Japan
  • Footnotes
    Commercial Relationships  A. Inoue, None; Y. Okada, None; A. Kitano, None; O. Yamanaka, None; S. Saika, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3400. doi:https://doi.org/
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    • Get Citation

      A. Inoue, Y. Okada, A. Kitano, O. Yamanaka, S. Saika; Effects of a Ppar-Gamma Ligand on Migration and Smad Activation in Corneal Epithelial Cell Line in vitro. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3400. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To determine whether or not troglitazone suppresses wound healing model in monolayer culture of the SV40- immortalized human corneal epithelial cell (HCEC) line. The perpxisome proliferation-activated receptor (PPAR) family consists of three members; PPAR-beta, -delta or -gamma which are involved in modulation of inflammatory cell function or cell proliferation during wound healing. Troglitazone is a ligand of PPAR-gamma.

Methods: : Efects of Troglitazon on HCEC migration were examined subsequent to wounding confluent monolayer cultures. Alamar blue staining evaluated the effects of Troglitazone (0-10 microM) on HCEC proliferation. Immunostaining determined Smad3. One to 3 hours after wounding, Troglitazone inhibits nuclear translocation of Smad3 near the wound.

Results: : Troglitazon suppressed wound-induced cell migration and proliferation. Injury-induced Smad nuclear translocalization was also suppressed by the compound in HCEC monolayer culture.

Conclusions: : Troglitazone suppresses wound healing in cultured HCEC sheet. These findings suggest that PPAR-gamma play a role in TGFb-driven corneal epithelial cell migration.

Keywords: wound healing • signal transduction • proliferation 
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