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A. Guzman, A. Crooke, A. Mediero, P. Alarma-Estrany, A. Peral, J. Pintor; Effect of Melatonin and Analogues on Corneal Wound Healing: Involvement of MT2 Melatonin Receptors. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3402. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
The aim of this study is to know the role of Melatonin, the MT3 agonist 5-MCA-NAT and MT2 agonist IIK7, have in the rate of re-epithelization after corneal wound healing as well as what melatonin receptor is involved in such process.
In wounded SIRC monolayers (establish rabbit corneal epithelial cell line) we studied the estimated migration rate (EMR) and estimated healing time (EHT) after treatment of wounds with melatonin, 5-MCA-NAT and IIK7 100 µM (n = 8). In the presence of melatonin 100 µM, we also assayed the effect the antagonists Luzindole (non-selective), DH97 (MT2) and Prazosin (MT3) (n = 8) had on the migration rate. A control wound was performed in each experiment, this being treated with buffer.
When we assayed both melatonin and IIK7 100 µM, the EMR was increased (6.65 ± 0.27 and 8.22 ± 0.39, respectively) in relation to the control (5.61 ± 0.54). This result was in accordance with a decrease in EHT in 5 and 10 hours for melatonin and IIK7, respectively. The MT3 selective agonist 5-MCA-NAT (100 µM) had no effect in migration rate (5.76 ± 0.16). In the presence of melatonin 100 µM, Luzindole 100µM, DH97 100 µM and Prazosin 1 µM produced a reduction in EMR (5.37 ± 1.09, 0.82 ± 0.45 and 2.73 ± 0.37 respectively) with an increase in EHT (9 hours, no apparent healing and 32 hours respectively). In the presence of 5-MCA-NAT 100 µM, DH97 100 µM and Corynanthine 25 µM produced a delay in EMR with an increased in EHT (2.93 ±0.85 and 70 hours for DH97 100 µM and 1.89 ± 0.38 and 60 hours for Corynanthine 25 µM), while Prazosin 1 µM had no effect on EMR (5.20 ± 0.30).
The increase of rate that melatonin exerts in corneal epithelial cell migration is due mainly to the activation of a MT2 receptor melatonin receptor.
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