May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Effect of Melatonin and Analogues on Corneal Wound Healing: Involvement of MT2 Melatonin Receptors
Author Affiliations & Notes
  • A. Guzman
    School of Optics. Complutense University, Madrid, Spain
    Biochemistry,
  • A. Crooke
    School of Optics. Complutense University, Madrid, Spain
    Biochemistry,
  • A. Mediero
    School of Optics. Complutense University, Madrid, Spain
    Biochemistry,
  • P. Alarma-Estrany
    School of Optics. Complutense University, Madrid, Spain
    Biochemistry,
  • A. Peral
    School of Optics. Complutense University, Madrid, Spain
    Optics,
  • J. Pintor
    School of Optics. Complutense University, Madrid, Spain
    Biochemistry,
  • Footnotes
    Commercial Relationships  A. Guzman, None; A. Crooke, None; A. Mediero, None; P. Alarma-Estrany, None; A. Peral, None; J. Pintor, None.
  • Footnotes
    Support  This study is supported by grants numbers SAF2004-06119-C02-01, SAF2007-60835, BM05-102-0 from Fundacion La Caixa and PR1/07-14890 from Universidad Complutense de Madrid.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3402. doi:https://doi.org/
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      A. Guzman, A. Crooke, A. Mediero, P. Alarma-Estrany, A. Peral, J. Pintor; Effect of Melatonin and Analogues on Corneal Wound Healing: Involvement of MT2 Melatonin Receptors. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3402. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The aim of this study is to know the role of Melatonin, the MT3 agonist 5-MCA-NAT and MT2 agonist IIK7, have in the rate of re-epithelization after corneal wound healing as well as what melatonin receptor is involved in such process.

Methods: : In wounded SIRC monolayers (establish rabbit corneal epithelial cell line) we studied the estimated migration rate (EMR) and estimated healing time (EHT) after treatment of wounds with melatonin, 5-MCA-NAT and IIK7 100 µM (n = 8). In the presence of melatonin 100 µM, we also assayed the effect the antagonists Luzindole (non-selective), DH97 (MT2) and Prazosin (MT3) (n = 8) had on the migration rate. A control wound was performed in each experiment, this being treated with buffer.

Results: : When we assayed both melatonin and IIK7 100 µM, the EMR was increased (6.65 ± 0.27 and 8.22 ± 0.39, respectively) in relation to the control (5.61 ± 0.54). This result was in accordance with a decrease in EHT in 5 and 10 hours for melatonin and IIK7, respectively. The MT3 selective agonist 5-MCA-NAT (100 µM) had no effect in migration rate (5.76 ± 0.16). In the presence of melatonin 100 µM, Luzindole 100µM, DH97 100 µM and Prazosin 1 µM produced a reduction in EMR (5.37 ± 1.09, 0.82 ± 0.45 and 2.73 ± 0.37 respectively) with an increase in EHT (9 hours, no apparent healing and 32 hours respectively). In the presence of 5-MCA-NAT 100 µM, DH97 100 µM and Corynanthine 25 µM produced a delay in EMR with an increased in EHT (2.93 ±0.85 and 70 hours for DH97 100 µM and 1.89 ± 0.38 and 60 hours for Corynanthine 25 µM), while Prazosin 1 µM had no effect on EMR (5.20 ± 0.30).

Conclusions: : The increase of rate that melatonin exerts in corneal epithelial cell migration is due mainly to the activation of a MT2 receptor melatonin receptor.

Keywords: cornea: epithelium • wound healing • receptors: pharmacology/physiology 
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