May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Expression of Interleukin-1 Receptor Antagonist mRNA in Human Corneal Epithelium
Author Affiliations & Notes
  • J. M. Heur
    Cole Eye Institute, Cleveland, Ohio
  • S. S. Chaurasia
    Cole Eye Institute, Cleveland, Ohio
  • S. E. Wilson
    Cole Eye Institute, Cleveland, Ohio
  • Footnotes
    Commercial Relationships  J.M. Heur, None; S.S. Chaurasia, None; S.E. Wilson, None.
  • Footnotes
    Support  NIH Grants EY10056 and EY015638, Support from Research to Prevent Blindness
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3403. doi:https://doi.org/
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    • Get Citation

      J. M. Heur, S. S. Chaurasia, S. E. Wilson; Expression of Interleukin-1 Receptor Antagonist mRNA in Human Corneal Epithelium. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3403. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the expression of interleukin-1 receptor antagonist (IL-1 Ra) in ex vivo human corneal epithelium.

Methods: : Four samples of human ex vivo corneal epithelium were obtained from patients undergoing PRK. The epithelium was scraped and placed in mRNA isolation buffer (Qiagen). mRNA was isolated from each sample and rt-PCR was performed using oligo-dT primers. PCR was then performed on the cDNA products using primers specific for IL-1Ra. The PCR products were sequenced to verify that the amplified DNA fragments were IL-1Ra.

Results: : IL-1 Ra was expressed in all of the ex vivo corneal epithelium samples.

Keywords: cornea: epithelium • wound healing 
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