May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Topical Application of Novel Quinazolinone OC-10X Does Not Adversely Affect the Retina: Multifocal Electroretinogram and Visual Evoked Response Analysis
Author Affiliations & Notes
  • S. S. Samudre
    Physiological Sciences, Eastern Virginia Medical School, Norfolk, Virginia
  • F. A. Lattanzio, Jr.
    Physiological Sciences, Eastern Virginia Medical School, Norfolk, Virginia
  • A. Hosseini
    Physiological Sciences, Eastern Virginia Medical School, Norfolk, Virginia
  • P. B. Williams
    Physiological Sciences, Eastern Virginia Medical School, Norfolk, Virginia
  • Footnotes
    Commercial Relationships  S.S. Samudre, None; F.A. Lattanzio, Ocucure Therapeutics Inc., C; A. Hosseini, None; P.B. Williams, Ocucure Therapeutics Inc., C.
  • Footnotes
    Support  Ocucure Therapeutics Inc.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3415. doi:https://doi.org/
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    • Get Citation

      S. S. Samudre, F. A. Lattanzio, Jr., A. Hosseini, P. B. Williams; Topical Application of Novel Quinazolinone OC-10X Does Not Adversely Affect the Retina: Multifocal Electroretinogram and Visual Evoked Response Analysis. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3415. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Methods: : For MFERG analysis, contact lens electrodes and stimuli with a 254 non-scaled hexagonal pattern were used. For VER analysis, sub-dermal Pt needle electrodes were placed near the visual cortex and stimulated with 8x8 square pattern. Full-field ERG (FF) was measured with corneal electrodes. To demonstrate detection of retinal damage by MFERG over 30 laser spots were placed in opposing quadrants and monitored for 2 wk. In the Brown Norway rat AMD model, choroidal neovascularization was stimulated by applying 8 radial laser photocoagulation spots at a constant distance from the optic disk. In this AMD model, some rats were treated with 1.0% OC-10X or vehicle control three times a day for up to 6 wk. Other rats without laser damage were also treated with OC-10X for up to 6 wk to examine for toxic effects on retina and optic nerve. To demonstrate detection of laser damage by FF, laser damage was compared with intravitreal injection of NMDA (40 nM).

Results: : In the rat AMD model, MFERG identified laser damaged quadrants as areas of retinal dysfunction and was able to differentiate individual laser spot damage corresponding to approximately 5 non-responsive hexagons (NRH). There were no corresponding changes in VER or FF. Results were corroborated with fluorescein angiography and histology. NMDA treatment significantly reduced FF a-wave amplitude to 171±10.4 µV compared to the contralateral untreated eye (241±14.0 µV; p=0.03). Although in the untreated AMD rats, NRH progressed over a 2 wk period from 5 to 8 hexagons with ~20% decrease in N1 and P1 amplitude, this was not the case with OC-10X treatment. In normal rats, treatment with OC-10X for up to 6 wk had no adverse effect on FF a-, b-wave, oscillatory potential, or flicker response (amplitude or latency) or VER (P1, N1 and P2).

Conclusions: : In an AMD model, electroretinography can quantify the extent of damage to retina or optic nerve. By this measure sustained topical application of OC-10X was not toxic to either retina or optic nerve. In addition, OC-10X appeared to retard retinal damage.

Keywords: age-related macular degeneration • electroretinography: non-clinical • laser 
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