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R. G. Tytarenko, V. V. Lyzogubov, S. Kaliappan, N. S. Bora, P. S. Bora; Adiponectin Peptide Inhibits Proliferation of Endothelial Cells and Inhibits Vessel Growth in Mouse Model of Laser Induced CNV. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3431.
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© ARVO (1962-2015); The Authors (2016-present)
Adiponectin (APN) participates in regulation of cell proliferation, apoptosis and inhibits choroidal neovascularization (CNV). The aim of this study was to investigate the effect of APN peptide on expression of proliferating cells nuclear antigen (PCNA), APN and AdipoR1 in endothelial cells (EC) during laser induced mouse model of CNV.
CNV was induced by laser photocoagulation in C57BL/6 mouse with Argon laser and investigated on day 1, 3, 5 and 7 post-laser. Mice were treated with APN peptide or PBS daily until they were sacrificed. Formalin fixed and paraffin embedded serial sections of the eyes were investigated by immunohistochemistry for PCNA, APN and AdipoR1. ECs were detected with isolectin IB4. RT-PCR analysis of VEGF, AdipoR1 and APN was performed in the same groups
Intraperitoneal administration of APN peptide decreases PCNA expression in preexisting vessels of the choroids at day 1 but did not prevent migration of isolectin IB4 binding cells into the area of laser injury. APN peptide did not affect proliferation of the cells located in area of inflammation at the 3rd and the 5th day. At day 7, when new vessels actively form, PCNA expression decreased again under condition of APN peptide treatment. We also found decrease of vessel size at this time point.
We conclude that APN peptide inhibits CNV formation by acting via regulation of proliferating endothelial cells. Application of APN peptide may stop active vessel growth in patients with AMD.
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