May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Dietary -3 Polyunsaturated Fatty Acids Reverse Late Stage Pathologic Retinal Neovascularization
Author Affiliations & Notes
  • K. M. Connor
    Ophthalmology, Children's Hospital Boston - Harvard Medical, Boston, Massachusetts
  • C. M. Aderman
    Ophthalmology, Children's Hospital Boston - Harvard Medical, Boston, Massachusetts
  • K. L. Willett
    Ophthalmology, Children's Hospital Boston - Harvard Medical, Boston, Massachusetts
  • J. Chen
    Ophthalmology, Children's Hospital Boston - Harvard Medical, Boston, Massachusetts
  • O. Aspergren
    Ophthalmology, Children's Hospital Boston - Harvard Medical, Boston, Massachusetts
  • N. SALEM, Jr.
    Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, Bethesda, Maryland
  • J.-P. SanGiovanni
    Division of Epidemiology and Clinical Research, National Eye Institute, Bethesda, Maryland
  • E. Y. Chew
    Division of Epidemiology and Clinical Research, National Eye Institute, Bethesda, Maryland
  • L. E. H. Smith
    Ophthalmology, Children's Hospital Boston - Harvard Medical, Boston, Massachusetts
  • Footnotes
    Commercial Relationships  K.M. Connor, None; C.M. Aderman, None; K.L. Willett, None; J. Chen, None; O. Aspergren, None; N. Salem, None; J. SanGiovanni, None; E.Y. Chew, None; L.E.H. Smith, None.
  • Footnotes
    Support  EY008670, EY017017, EY14811, V.Kann Rasmussen Foundation, Alcon Research Award, Lew Wasserman Merit Award (L.E.H.S.); 5 T32 EY07145, 1 F32 EY017789 (K.M.C); Juvenile Diabetes Research Foundation (J.C)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3441. doi:https://doi.org/
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    • Get Citation

      K. M. Connor, C. M. Aderman, K. L. Willett, J. Chen, O. Aspergren, N. SALEM, Jr., J.-P. SanGiovanni, E. Y. Chew, L. E. H. Smith; Dietary -3 Polyunsaturated Fatty Acids Reverse Late Stage Pathologic Retinal Neovascularization. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3441. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Retinal neovascularization, a prevalent cause of blindness and is characterized by two critical phases, vessel loss followed by hypoxia-driven destructive neovascularization. Our previous studies demonstrated that a lifelong ω-3 versus an ω-6 PUFA diet protected mice from oxygen-induced retinopathy by preventing neovascularization through revascularization of hypoxic retina. While long term treatment with dietary ω-3 PUFAs is clearly beneficial in preventing retinopathy it remains unclear what effect late stage treatment with dietary ω-3 PUFAs would have on reversing retinal neovascularization. Our initial study showed that during the vessel loss phase of the disease (when mice are in high oxygen) dietary intake had no effect on vessel loss.

Methods: : In the oxygen-induced retinopathy model, C57Bl/6 mothers were fed (from postnatal day 0 (P0)) a diet enriched with ω-3 PUFA or a diet elevated in ω-6 PUFA. ω PUFA composition of the milk from the mothers reflected their diet. Alternatively, mice were treated with an ω-3 PUFA or ω-6 PUFA diet at P12 or P15, after the initial vessel loss phase of oxygen-induced retinopathy. We then analyzed the role these diets played in reversing late stage disease. Retinal RNA was also isolated for real-time PCR analysis.

Results: : As seen previously, mice on a lifelong ω-3 (versus ω-6) enriched PUFA diet had a significant increase in vessel re-growth as well as diminished neovascularization and a decrease in both macrophage markers and associated inflammatory molecules. In mice given the diets after vessel loss, no change in vessel re-growth (vaso-obliteration) was observed at P17. However, mice on a ω-3 PUFA diet were significantly protected (~50%) from the pathological neovascular stage of the disease compared to their ω-6 PUFA fed counterparts. Here no change in macrophage markers was observed. However in the ω-3 PUFA fed mice there was a significant decrease (40%) in inflammatory molecules.

Conclusions: : This data indicates that ω-3 PUFAs can reverse the pathological neovascularization phase of retinopathy after it has been initiated this is associated with a dampening of the inflammatory response.

Keywords: retinal neovascularization • lipids • inflammation 
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