May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Gene Profiles of Omega-3 and Omega-6 PUFA Fed Mice With Retinopathy
Author Affiliations & Notes
  • C. M. Aderman
    Ophthalmology, Children's Hospital Boston, Boston, Massachusetts
  • K. M. Connor
    Ophthalmology, Children's Hospital Boston, Boston, Massachusetts
  • J. Chen
    Ophthalmology, Children's Hospital Boston, Boston, Massachusetts
  • K. L. Willett
    Ophthalmology, Children's Hospital Boston, Boston, Massachusetts
  • O. P. Aspegren
    Ophthalmology, Children's Hospital Boston, Boston, Massachusetts
  • J. P. SanGiovanni
    Division of Epidemiology and Clinical Research, National Eye Institute/NIH, Bethesda, Maryland
  • E. Y. Chew
    Division of Epidemiology and Clinical Research, National Eye Institute/NIH, Bethesda, Maryland
  • N. Salem, Jr.
    Laboratory of Membrane Biochemistry and Biophysics, NIH/National Institute on Alcohol Abuse and Alcoholism, Bethesda, Maryland
  • L. E. H. Smith
    Ophthalmology, Children's Hospital Boston, Boston, Massachusetts
  • Footnotes
    Commercial Relationships  C.M. Aderman, None; K.M. Connor, None; J. Chen, None; K.L. Willett, None; O.P. Aspegren, None; J.P. SanGiovanni, None; E.Y. Chew, None; N. Salem, None; L.E.H. Smith, None.
  • Footnotes
    Support  EY008670, EY017017, EY14811, V. Kann Rasmussen Foundation, P01 HD18655, Alcon Research Institution Award (LEHS), 1 F32 EY017789 (KMC), Juvenile Diabetes Research Foundation fellowship (JC)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3447. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      C. M. Aderman, K. M. Connor, J. Chen, K. L. Willett, O. P. Aspegren, J. P. SanGiovanni, E. Y. Chew, N. Salem, Jr., L. E. H. Smith; Gene Profiles of Omega-3 and Omega-6 PUFA Fed Mice With Retinopathy. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3447. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : We have previously reported that mice with elevated levels of omega-3 polyunsaturated fatty acids, through either dietary or genetic means, are protected from retinopathy through enhanced vessel regrowth. Mice on a diet enriched in omega-3 fatty acids had decreased levels of microglial-derived TNF-alpha in a model of oxygen induced retinopathy. To further investigate the protective properties of omega-3 fatty acids in pathological neovascularization, we performed Illumina microarray analysis on retinas from mice on diets rich in either omega-3 or omega-6 polyunsaturated fatty acids (PUFAs).

Methods: : Beginning at postnatal day 0 (P0), mothers were fed diets enriched with either omega-3 or omega-6 PUFAs. To induce vessel loss, and subsequent pathological neovascularization, nursing mothers and pups were exposed to 75% oxygen from P7 to P12 and returned to room air and sacrificed at P17. One eye from each mouse was isolated and lectin-stained for quantification of neovascularization and vaso-obliteration. Contralateral retinas from each group were isolated and flash frozen using RNase-free techniques. Total RNA was extracted and prepared for Illumina microarray analysis using the MouseRef-6 chip. Data was acquired using the Illumina BeadStudio software and further analysis was performed using Significance Analysis of Microarray (SAM), Gene Set Enrichment Analysis (GSEA), and J-Express Pro 2.7 software. A set of differentially expressed genes were validated with real-time PCR.

Results: : In line with previous studies, both vaso-obliteration (VO) and neovascularization (NV) were more severe in retinas of omega-6 fed mice (%VO: 19.0 ± 1.0; %NV: 10.2 ± 0.7) than in retinas of their omega-3 fed counterparts (%VO: 14.7 ± 1.5, p < 0.05; %NV: 2.9 ± 0.5, p < 0.001) following oxygen exposure. Contralateral retinas from these mice were used for Illumina microarray analysis, which showed a 2-8 fold upregulation of activated macrophage, inflammation, angiogenesis, and adhesion markers in omega-6 fed mice.

Conclusions: : These findings suggest that upregulation of adhesion markers in omega-6 fed mice may be responsible for recruiting inflammatory cells and activated macrophages in the retina, resulting in an increased production of pro-angiogenic signals and more severe pathological neovascularization.

Keywords: neovascularization • retinopathy of prematurity • inflammation 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×