May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
UV-Induced Damage and Protective Effects of Catechin in Cultured Human Retinal Pigment Epithelial Cells
Author Affiliations & Notes
  • Y. Lee
    Department of Ophthalmology, Chosun University College of Medicine, Gwangju, Republic of Korea
  • M. Jung
    Department of Ophthalmology, Soonchunhyang University College of Medicine, Seoul, Republic of Korea
  • S. Kim
    Department of Ophthalmology, Soonchunhyang University College of Medicine, Seoul, Republic of Korea
  • J. Koh
    Department of Ophthalmology, Chosun University College of Medicine, Gwangju, Republic of Korea
  • Footnotes
    Commercial Relationships  Y. Lee, None; M. Jung, None; S. Kim, None; J. Koh, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3532. doi:https://doi.org/
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      Y. Lee, M. Jung, S. Kim, J. Koh; UV-Induced Damage and Protective Effects of Catechin in Cultured Human Retinal Pigment Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3532. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate the protective effects of Epigallocatechin gallate (EGCG) against UV irradiation in cultured human retinal pigment epithelial (RPE) cells.

Methods: : UV irradiation was produced by a UV lamp for 30 seconds with an irradiance of 3.3 mW/cm2. After 5 minutes and 1 hour, we administered different concentrations of EGCG (0, 5, 10, 15, 25, 50, 100 uM). The cell count was determined under a microscope using a counting chamber and the cell activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.

Results: : The cell count of cultured human RPE cells after UV irradiation was markedly increased in the EGCG administration group, compared with the non-administrated group. The cell activity of the cultured human RPE cells after UV irradiation was markedly increased in the EGCG administration group and was increased in a dose-dependent way as determined by the MTT assay.

Conclusions: : The administration of EGCG increased the cell count and the cell activity after UV irradiation in cultured human retinal pigment epithelial cells; this suggests that EGCG provided protection against UV damage in cultured human retinal pigment epithelial cells.

Keywords: retinal pigment epithelium • radiation damage: light/UV • cell survival 
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