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S. A. Cicero, M. A. Dyer; Transdifferentiation of Murine Ciliary Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3574.
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© ARVO (1962-2015); The Authors (2016-present)
To fully characterize the sphere forming cells arising from the adult ciliary epithelium.
Ciliary epithelia (CE) and spheres derived from ciliary epithelia were analyzed by TEM, real-time RT-PCR, dual beam electron microscopy (FIB) and immuno-fluorescence. Cultures of differentiated spheres were analyzed by immuno-fluorescence. Cell cycle analysis was done with the addition of 3H-thymidine. Adult retina, RPE, iris, and CE, as well as adult brain SVZ (sub-ventricular zone) and neurospheres derived from the adult SVZ, were used as controls.
Data from our cell cycle labeling studies as well as dual beam FIB indicates that the proliferative cells maintain their differentiated ciliary phenotype. Cells in CE-derived spheres had junctional complexes such as tight junctions, similar to the CE from which they were derived. CE-derived spheres expressed genes that are found in the CE and few retinal markers. Differentiated cultures expressed genes of both the CE and the retina.
The cells that form spheres in vitro more closely resemble the cells of the ciliary epithelium than those of the retina. The data are more consistent with a transdifferentiation mechanism rather than a stem cell mechanism.
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