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S. M. Nehls, S. H. Brounts, T. A. Levitt, C. Schmiedt, J. McAnulty, C. J. Murphy; In vitro Cytotoxicity of Tissue Adhesive Products. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3612.
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The use of tissue adhesives in ocular surgical procedures is increasing, however, little is known about the comparative cytotoxic effects of available products. This study compares the relative in vitro cytotoxicity of aqueous leachable products of six different tissue adhesives.
We studied tissue adhesives of varied types including polyethylene glycol polymer based, investigational mussel adhesive protein based, cross-linked bovine serum albumin based, and three cyanoacrylates of differing chemical composition (2-octylcyanoacrylate, methoxypropyl cyanoacrylate and isobutyl cyanoacrylate). Each adhesive was placed in cell culture media for 24 hours at 37oC and the supernatant was then used to determine the relative toxicity of leachables from the adhesive. Five cultured human cell lines (keratinocytes, corneal epithelial cells, dermal fibroblasts, corneal fibroblasts, and arterial smooth muscle cells) were exposed for 24 hours to the adhesive treated supernatant in dilutions ranging from neat to 1:1000. Relative cytotoxicity was determined using a Calcein AM fluorescence-based microplate assay for cell viability. Statistical comparisons were made utilizing a paired T-test with significance set at a P-value ≤0.05.
Concentration dependent cytotoxicity was seen with all adhesive products. In general, the greatest toxicity was observed with the cyanoacrylate adhesives and the least toxicity was observed with the polyethylene glycol based adhesive. Intermediate toxicity was seen with the other adhesive products.
Aqueous leachable materials from six tissue adhesives products were associated with different levels of in vitro cytotoxicity, dependent on the adhesive and the cell line tested.
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