May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Early Elevation of OX2 Antigen and OX2 Receptor in TNF--Induced Optic Nerve Neurodegeneration
Author Affiliations & Notes
  • H. Takeda
    Ophthalmology, St Marianna Univ Sch of Med, Kawasaki-shi, Japan
  • Y. Kitaoka
    Ophthalmology, St Marianna Univ Sch of Med, Kawasaki-shi, Japan
  • H. Fujino
    Ophthalmology, St Marianna Univ Sch of Med, Kawasaki-shi, Japan
  • Y. Hayashi
    Ophthalmology, St Marianna Univ Sch of Med, Kawasaki-shi, Japan
  • Y. Munemasa
    Ophthalmology, Jules Stein Eye Institute, UCLA, Los Angeles, California
  • S. Ueno
    Ophthalmology, St Marianna Univ Sch of Med, Kawasaki-shi, Japan
  • Footnotes
    Commercial Relationships  H. Takeda, None; Y. Kitaoka, None; H. Fujino, None; Y. Hayashi, None; Y. Munemasa, None; S. Ueno, None.
  • Footnotes
    Support  Grants-in-aid for scientific reserch of Japanese Ministry of Education, Culture, Sports, Science and Technology(No.18791303)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3692. doi:https://doi.org/
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    • Get Citation

      H. Takeda, Y. Kitaoka, H. Fujino, Y. Hayashi, Y. Munemasa, S. Ueno; Early Elevation of OX2 Antigen and OX2 Receptor in TNF--Induced Optic Nerve Neurodegeneration. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3692. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate the distribution of OX2 and its receptor within the optic nerve and to investigate the involvement of OX2/OX2R in TNF-α-induced optic nerve degeneration.

Methods: : Eight weeks old male Wistar rats were treated with intravitreous injection of 10 ng TNF-α into one eye. PBS was administered as a control. Eyes were removed at 24 hr, 1 or 2 weeks after the injection. The protein levels of OX2 and OX2R in the optic nerve were examined by Western blot analysis. Expression of OX2 and OX2R were detected by immunohistochemistry.

Results: : Compared to control eyes, the protein levels of OX2 and OX2R were significantly increased 24 hr after TNF-α injection. Immunohistochemistry showed substantial increase in GFAP immunoreactivity in TNF-α treated eyes. Furthermore, our double-labeling immunohistochemical study showed that OX2 was co-localized with GFAP, but not neurofilament 24 hr after TNF-α injection.

Conclusions: : We found that OX2 and its receptor were expressed in astrocytes, suggesting that OX2-OX2R pathway may play an important role as a glial response in TNF-α-induced optic nerve degeneration.

Keywords: optic nerve • astrocyte • neuroprotection 
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