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V. S. Liarakos, M. Papathanassiou, P. Theodossiadis, A. Rouvas, M. Douvali, E. Papastergiopoulou, I. A. Vergados; Subconjunctival Ranibizumab and Bevacizumab Inhibit Corneal Neovascularization in an Animal Model. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3743.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the effect of two anti-VEGF agents, Bevacizumab and Ranibizumab, on experimentally induced corneal neovascularization (corneal NV).
Eighteen N.Zealand white rabbits were randomly and equally divided in 6 groups. A model of corneal NV secondary to alkali burn was applied to groups 1-4. A single injection was administered subconjunctivally 2mm from the limbus as follows: 3.75mg of Bevacizumab on day 1 (group 1) or day 14 (group 2); 0.5mg of Ranibizumab on day 1 (group 3); a sham injection of 0.15cc BSS on day 1 (group 4). Groups 5 and 6 were left uncauterized but were treated with 3.75mg of Bevacizumab and 0.5mg of Ranibizumab respectively. Follow-up ended in 28 days. Every procedure was conducted in compliance with the ARVO statement for the use of animals in ophthalmic and vision research.
Corneal NV in groups 1-4 on day 28 was 4.7±3.1%, 13.3±2.3%, 7.3±6.7% and 34.7±9.3% of the corneal surface respectively. Corneal NV was found to be significantly restricted in the treated groups compared to the untreated, both on day 7 as well as day 28 (p<0.05; one-way ANOVA). Bevacizumab treatment on day 1 showed best results. Early administration of Bevacizumab proved to be more effective than late treatment in the first as well as fourth week (p=0.037 and p=0.046 respectively; Mann-Whitney U test). Bevacizumab provided slightly better results compared to Ranibizumab; difference was statistically significant in the first but not in the fourth week (p=0.037 and p=0.658 respectively; Mann-Whitney U test). Induced corneal scarring remained relatively stable during the 4 weeks (39.6±3.5%), with no significant difference between cauterized groups (p>0.05; one-way ANOVA). No side-effects were recorded.
Early subconjunctival administration of the anti-VEGF agents Bevacizumab and Ranibizumab could successfully inhibit corneal neovascularization, without any side effects. Treatment did not affect the course of corneal scarring.
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