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S. M. Delgado, F. Manns, W. Lee, I. Nose, A. Amelinckx, E. Arrieta-Quintero, D. Nankivil, D. Borja, R. Jain, J.-M. Parel; Compact Scheiner System for Optical Power Assessment of ex vivo Crystalline Lenses. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3790.
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© ARVO (1962-2015); The Authors (2016-present)
To measure the refractive power of ex vivo animal crystalline lenses using a compact, portable Scheiner system.
The beam of a 635nm laser diode is transmitted thru a multimode fiber to an infinity collimator and an opaque disc with four optically centered equidistant pinholes (200um dia at 2 or 3mm center-to-center) mounted 240mm above a stand holding the prepared crystalline lens sample which is immersed in a Petri dish filled with DMEM. A CCD mounted on the measuring arm of a digital vernier (#570-244 Mitutoyo, Tokyo, Japan) permits visualization of the four converging beams. The focal length of the sample lens is determined by finding the intersection point of the four beams. The system was calibrated in air using a set of glass lenses of back focal lengths ranging from 12.03 to 70.42mm (Edmund Optics). Enucleated whole eyes were bonded to a plastic ring using cyanoacrylate adhesive and dissected leaving a transverse anterior section containing the crystalline lens, zonular apparatus, ciliary body and a scleral rim attached to the ring. The refractive power of 3 human (range 55-60 years, <3 days postmortem), 7 cynomolgus (range 3-9years, <1 day postmortem) and 1 baboon (5 years old, 3hrs postmortem) lenses were measured three times each.
The accuracy and precision of the Scheiner system evaluated on the glass lenses ranged from -0.60+/-0.2D to +1.12+/-0.57D. Refractive powers of natural lenses were: human: 21.71+/- 0.39D to 23.14+/-0.18D, cynomolgus: 49.40+/-0.78D to 55.03D+/-0.65D and baboon: 43.53+/-1.25D.
The portable Scheiner system allows rapid and precise dioptric power determination of human and animal ex vivo lenses supported by an intact zonular framework.Support: NIH: 2R01EY14225, 5F31EY15395, P30EY14801 (Center Grant); the Florida Lions Eye Bank; Advanced Medical Optics Inc, Santa Ana, CA; an unrestricted grant from Research to Prevent Blindness; the Australian Federal Government CRC Scheme through the Vision Cooperative Research Centre, Sydney Australia; Henri and Flore Lesieur Foundation.
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