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K. Kawai, H. Oohashi, T. Suzuki; Effect of Anti-Glaucoma Drugs on Inflammatory Cytokine Production by Human and Murine Peripheral Blood Mononuclear Cells. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3815.
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We previously reported that suppression of inflammation after surgery leads to suppression of postoperative elevation of intraocular pressure. We investigated human and murine peripheral blood mononuclear cells to find a drug that suppresses inflammation from among the anti-glaucoma drugs used to lower intraocular pressure.
Peripheral blood mononuclear cells (PBMCs) prepared from healthy males and BALB/C mice were suspended in RPMI 1640 culture medium containing 10% FBS, each test drug (beta-antagonists ; timolol, betaxolol, levobunolol, nipradilol,carteolol carbonic anhydrase inhibitor (CAI) ; dorzolamide prostaglandin (PG) ; isopropyl unoprostone, latanoprost ) was added, and they were cultured in a CO2 incubator (set to 37°C and 5% CO2) for 30 minutes. Then, lipopolysaccharide (LPS) was added and they were cultured for a given period of time, after which TNF-alpha or IL-6 levels in the culture medium were determined by ELISA.
The beta-stimulant salbutamol inhibited production of TNF-alpha, which is an inflammatory cytokine that comes from human and murine PBMCs. Carteolol, which has an intrinsic sympathomimetic action (ISA), inhibited production of TNF-alpha or IL-6 from PBMCs in a dose-dependent manner. On the other hand, an inhibitory action was not observed in the case of timolol, betaxolol, levobunolol, and nipradilol, which are beta-antagonists with no intrinsic sympathomimetic action. Dorzolamide, isopropyl unoprostone and latanoprost had almost no effect on production of inflammatory cytokines.
It was demonstrated that carteolol exhibited an inhibitory action on production of inflammatory cytokines from PBMCs because of its intrinsic sympathomimetic action. Moreover, it was shown that CAI and PG drugs had almost no effect on inflammatory cytokine production.
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