May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Evaluation of Ocular Surface Toxicity in Rabbits Following Exposure to Preservative-Free Tafluprost, Commercially Available Latanoprost and 0.02% Bac
Author Affiliations & Notes
  • F. Baudouin
    Toxicology, Faculty of Pharmaceutical and Biological Sciences, Paris, France
    INSERM, UMR S 872, Cordeliers Biomedical Institute, University Paris Descartes, Paris, France
  • H. Liang
    Toxicology, Faculty of Pharmaceutical and Biological Sciences, Paris, France
    INSERM, UMR S 872, Cordeliers Biomedical Institute, University Paris Descartes, Paris, France
  • A. Pauly
    Toxicology, Faculty of Pharmaceutical and Biological Sciences, Paris, France
    INSERM, UMR S 872, Cordeliers Biomedical Institute, University Paris Descartes, Paris, France
  • E. Brasnu
    INSERM, UMR S 872, Cordeliers Biomedical Institute, University Paris Descartes, Paris, France
    Ophthalmology, Quinze-Vingts National Ophthalmology Hospital, Paris, France
  • J.-M. Warnet
    Toxicology, Faculty of Pharmaceutical and Biological Sciences, Paris, France
    INSERM, UMR S 872, Cordeliers Biomedical Institute, University Paris Descartes, Paris, France
  • C. Baudouin
    INSERM, UMR S 872, Cordeliers Biomedical Institute, University Paris Descartes, Paris, France
    Ophthalmology, Quinze-Vingts National Ophthalmology Hospital, Paris, France
  • Footnotes
    Commercial Relationships  F. Baudouin, None; H. Liang, None; A. Pauly, None; E. Brasnu, None; J. Warnet, None; C. Baudouin, santen, C.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3819. doi:https://doi.org/
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      F. Baudouin, H. Liang, A. Pauly, E. Brasnu, J.-M. Warnet, C. Baudouin; Evaluation of Ocular Surface Toxicity in Rabbits Following Exposure to Preservative-Free Tafluprost, Commercially Available Latanoprost and 0.02% Bac. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3819. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The preservative benzalkonium chloride (BAK) has shown dose-dependent toxic effects on ocular surface. BAK-free prostaglandin analogues are in development for glaucoma treatment. This study evaluates the conjunctival and corneal reactions in rabbits of the preservative-free tafluprost, a new prostaglandin analogue.

Methods: : Four groups of 6 male New Zealand albino rabbits received preservative-free tafluprost 0.0015%; a commercially available solution of latanoprost 0.005% containing 0.02% BAK; 0.02% BAK alone or phosphate-buffered saline (PBS) 15 times at 5-min intervals. Ocular surface toxicity was examined using in vivo confocal microscopy, flow cytometry on conjunctival impression cytology and classical cytological and immunohistological methods.

Results: : Clinical observation using slit-lamp revealed the highest toxicity for BAK alone and latanoprost, while preservative-free tafluprost showed similar results to PBS. In vivo confocal microscopy demonstrated severe damages in the epithelium and marked inflammatory infiltration with latanoprost and BAK but normal structures with preservative-free tafluprost and PBS. Flow cytometry showed a higher expression of CD45 and TNFR1 in latanoprost or BAK-instilled groups, compared with preservative-free tafluprost and PBS groups; latanoprost induced fewer positive cells compared with BAK alone. On cryosections, infiltration of CD45+ inflammatory cells and TUNEL+ apoptotic cells was higher in limbal and conjunctival areas of latanoprost and BAK-treated eyes, compared with preservative-free tafluprost and PBS-treated eyes.

Conclusions: : Rabbit corneoconjunctival surface showed better tolerance when treated with preservative-free tafluprost compared with commercially available latanoprost or 0.02% BAK-solution.

Keywords: drug toxicity/drug effects • flow cytometry • microscopy: confocal/tunneling 
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