May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Neurite Arborization and Mosiac Formation in the Mouse Retina Requires the Down Syndrome Cell Adhesion Molecule
Author Affiliations & Notes
  • P. G. Fuerst
    Burgess Lab, The Jackson Laboratory, Bar Harbor, Maine
  • A. Koizumi
    Massachusetts General Hospital, Harvard University, Massachusetts
  • R. H. Masland
    Massachusetts General Hospital, Harvard University, Massachusetts
  • R. W. Burgess
    Burgess Lab, The Jackson Laboratory, Bar Harbor, Maine
  • Footnotes
    Commercial Relationships  P.G. Fuerst, None; A. Koizumi, None; R.H. Masland, None; R.W. Burgess, None.
  • Footnotes
    Support  NIH Grant EY016031
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3842. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      P. G. Fuerst, A. Koizumi, R. H. Masland, R. W. Burgess; Neurite Arborization and Mosiac Formation in the Mouse Retina Requires the Down Syndrome Cell Adhesion Molecule. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3842.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose:
 

The molecular cues that direct these processes in the vertebrate retina are not known.

 
Methods:
 

We have identified a mutant mouse model in which organization of the retina is disrupted.

 
Results:
 

Some types of retinal amacrine cells from mice with a spontaneous mutation in Dscam, a gene encoding an Ig-superfamily member adhesion molecule, have defects in the arborization of processes and the spacing of cell bodies. In the mutant retina, cells that would normally express Dscam have hyperfasciculated processes, preventing them from creating an orderly arbor. The cell bodies of these neurons are randomly distributed or pulled into clumps rather than being spaced in regular mosaics. Retinal ganglion cells also have defects in spacing and dendritic arborization. Ganglion cell axonal pathfinding is normal, although axonal remodeling is observed after eye opening.

 
Conclusions:
 

Our results indicate that mouse DSCAM mediates isoneuronal selfavoidance for arborization, and heteroneuronal self-avoidance within specific cell types to prevent fasciculation and to preserve mosaic spacing. These functions are highly analogous to those of Drosophila Dscam and Dscam2, and are necessary for establishing orderly retinal circuitry.  

 
Keywords: retinal connections, networks, circuitry • amacrine cells • retinal development 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×