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Y. Wu, Y. Guan, L. Cui, J.-Y. Xu, C. Li, X. Zhong, W. Li, G.-T. Xu; Transplantation of Marrow Stromal Cells to Rescue Retinal Cells in SI-Induced Retinal Degeneration in Rats. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3868. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the therapeutic effects of the subretinal transplantation of rat Marrow Stromal derived Cells (rMSC) in rat retinal degeneration model induced by sodium iodate (SI).
Retinal degeneration in rats was induced by single intraveneous injection of SI (50 mg/kg). rMSC were isolated from bone marrow aspirates from the femurs of male adult SD rats, then cultured in Blood-Retinal Barrier Determination (BRBD) medium for in vitro differentiation. Specific signaling molecules required for Blood Vessel Endothelium (BVE) specification were added into the BRBD medium. rMSCs were used for transplantation as donor cells in the SI treated rats. The effects of transplantation were evaluated by funduscopy, Fundus Fluorescein Angiography (FFA) and electroretinogram (ERG), from 1 to 9 weeks following the operation. The transplantation efficiency of the donor cells was determined by their survival, integration, and differentiation into retinal cells at the same intervals.
Retinal pigment epithelium (RPE) and photoreceptors were damaged after the treatment of SI. The severity of the damage depends on the dosage of SI and the time of the treatment. After rMSCs transplantation, CM-DiI labeled donor cells could be observed, scattered in the retina, and adopted BRB-like morphologies. In the retinal degeneration rats receiving rMSC transplantation, the retinal function improved as shown by ERG, for at least 9 weeks after the transplantation.
By using the standard retinal degeneration model induced by SI treatment a medium-term efficacy of rMSC transplantion was observed. Our data showed that the transplanted rMSCs survived, integrated and differentiated in recipient subretinal space. MSCs may be used as a reliable resource of donor cells for autotransplantation in the treatment of retinal degeneration.
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