May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Transplantation of Marrow Stromal Cells to Rescue Retinal Cells in SI-Induced Retinal Degeneration in Rats
Author Affiliations & Notes
  • Y. Wu
    Lab Clinical Visual Sci, Shanghai Inst Biol Sci. CAS JTU, Shanghai, China
    Key Laboratory of Stem Cell Biology, SIBS, CAS, Shanghai, China
  • Y. Guan
    Lab Clinical Visual Sci, Shanghai Inst Biol Sci. CAS JTU, Shanghai, China
    Key Laboratory of Stem Cell Biology, SIBS, CAS, Shanghai, China
  • L. Cui
    Lab Clinical Visual Sci, Shanghai Inst Biol Sci. CAS JTU, Shanghai, China
    Key Laboratory of Stem Cell Biology, SIBS, CAS, Shanghai, China
  • J.-Y. Xu
    Lab Clinical Visual Sci, Shanghai Inst Biol Sci. CAS JTU, Shanghai, China
    Key Laboratory of Stem Cell Biology, SIBS, CAS, Shanghai, China
  • C. Li
    Key Laboratory of Stem Cell Biology, SIBS, CAS, Shanghai, China
  • X. Zhong
    Key Laboratory of Stem Cell Biology, SIBS, CAS, Shanghai, China
  • W. Li
    Lab Clinical Visual Sci, Shanghai Inst Biol Sci. CAS JTU, Shanghai, China
    Dept. of Ophthalmology, Drexel University College of Medicine, Philadelphia, Pennsylvania
  • G.-T. Xu
    Lab Clinical Visual Sci, Shanghai Inst Biol Sci. CAS JTU, Shanghai, China
    Key Laboratory of Stem Cell Biology, SIBS, CAS, Shanghai, China
  • Footnotes
    Commercial Relationships  Y. Wu, None; Y. Guan, None; L. Cui, None; J. Xu, None; C. Li, None; X. Zhong, None; W. Li, None; G. Xu, None.
  • Footnotes
    Support  (1) Shanghai Science and Technology Committee Grant (06DJ1400001); (2) Chinese Academy of Sciences (KSCX2-YW-R-46)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3868. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Y. Wu, Y. Guan, L. Cui, J.-Y. Xu, C. Li, X. Zhong, W. Li, G.-T. Xu; Transplantation of Marrow Stromal Cells to Rescue Retinal Cells in SI-Induced Retinal Degeneration in Rats. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3868.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To investigate the therapeutic effects of the subretinal transplantation of rat Marrow Stromal derived Cells (rMSC) in rat retinal degeneration model induced by sodium iodate (SI).

Methods: : Retinal degeneration in rats was induced by single intraveneous injection of SI (50 mg/kg). rMSC were isolated from bone marrow aspirates from the femurs of male adult SD rats, then cultured in Blood-Retinal Barrier Determination (BRBD) medium for in vitro differentiation. Specific signaling molecules required for Blood Vessel Endothelium (BVE) specification were added into the BRBD medium. rMSCs were used for transplantation as donor cells in the SI treated rats. The effects of transplantation were evaluated by funduscopy, Fundus Fluorescein Angiography (FFA) and electroretinogram (ERG), from 1 to 9 weeks following the operation. The transplantation efficiency of the donor cells was determined by their survival, integration, and differentiation into retinal cells at the same intervals.

Results: : Retinal pigment epithelium (RPE) and photoreceptors were damaged after the treatment of SI. The severity of the damage depends on the dosage of SI and the time of the treatment. After rMSCs transplantation, CM-DiI labeled donor cells could be observed, scattered in the retina, and adopted BRB-like morphologies. In the retinal degeneration rats receiving rMSC transplantation, the retinal function improved as shown by ERG, for at least 9 weeks after the transplantation.

Conclusions: : By using the standard retinal degeneration model induced by SI treatment a medium-term efficacy of rMSC transplantion was observed. Our data showed that the transplanted rMSCs survived, integrated and differentiated in recipient subretinal space. MSCs may be used as a reliable resource of donor cells for autotransplantation in the treatment of retinal degeneration.

Keywords: transplantation • retinal pigment epithelium • age-related macular degeneration 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×