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J. I. Lim, C. Spee, D. R. Hinton; Comparison of the Presence of Hypoxic-Inducible Factor 1-alpha (HIF-1 ) in Diabetic Membranes With Non-Diabetic Membranes. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3874.
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To determine the pattern and extent of expression of hypoxic-inducible factor alpha (HIF-1α) protein in diabetic membranes and non-diabetic epiretinal membranes.
Twelve patients with diabetic retinopathy and nine patients with idiopathic epiretinal membranes underwent pars plana vitrectomy (PPVx). Three postmortem normal human eyes and normal rabbit eyes served as negative controls. The specimens and controls underwent immunofluorescence staining for HIF-1α. DAPI ( 4’,6’ diamidino-2-phenylindole) was used to label the cell nuclei within the membranes. Laser scanning confocal microscopy was used to determine the presence of HIF-1α within the tissues. The degree of immunofluorescence for HIF-1α (1+, 2+, 3+ scale) and the location of immunoreactivity were determined for each specimen and control.
Eleven of 12 (92 %) diabetic preretinal membranes were immunoreactive for HIF-1α and most had intense (2-3+) cytoplasmic positivity with occasional, focal nuclear positivity. Five of 9 (55 %) non-diabetic, epiretinal membranes were positive for HIF-1α with significantly weaker cytoplasmic immunoreactivity (1-2 +) and occasional, punctuate nuclear staining. All post-mortem sections and rabbit controls were negative for HIF-1α.
Immunoreactive HIF-1α protein is identified in diabetic neovascular membranes more often and more intensely than in non-diabetic epiretinal membranes.
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