May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Vascular Endothelial Growth Factor Levels in Aqueous, Vitreous, Blood and Subretinal Fluid in Patients With Stage V Retinopathy of Prematurity
Author Affiliations & Notes
  • H. Ruiz-Garcia
    Retina, Asociacion para Evitar la Ceguera en Mexico, IAP, Mexico City, Mexico
  • R. Velez-Montoya
    Retina, Asociacion para Evitar la Ceguera en Mexico, IAP, Mexico City, Mexico
  • O. Ustariz-Gonzalez
    Retina, Asociacion para Evitar la Ceguera en Mexico, IAP, Mexico City, Mexico
  • M. Gordon-Angelozzi
    Retina, Asociacion para Evitar la Ceguera en Mexico, IAP, Mexico City, Mexico
  • G. Garcia-Aguirre
    Retina, Asociacion para Evitar la Ceguera en Mexico, IAP, Mexico City, Mexico
  • M. E. Torres-Soriano
    Retina, Asociacion para Evitar la Ceguera en Mexico, IAP, Mexico City, Mexico
  • V. Kon-Jara
    Retina, Asociacion para Evitar la Ceguera en Mexico, IAP, Mexico City, Mexico
  • J. L. Guerrero-Naranjo
    Retina, Asociacion para Evitar la Ceguera en Mexico, IAP, Mexico City, Mexico
  • H. Quiroz-Mercado
    Retina, Asociacion para Evitar la Ceguera en Mexico, IAP, Mexico City, Mexico
  • Footnotes
    Commercial Relationships  H. Ruiz-Garcia, None; R. Velez-Montoya, None; O. Ustariz-Gonzalez, None; M. Gordon-Angelozzi, None; G. Garcia-Aguirre, None; M.E. Torres-Soriano, None; V. Kon-Jara, None; J.L. Guerrero-Naranjo, None; H. Quiroz-Mercado, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3877. doi:
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      H. Ruiz-Garcia, R. Velez-Montoya, O. Ustariz-Gonzalez, M. Gordon-Angelozzi, G. Garcia-Aguirre, M. E. Torres-Soriano, V. Kon-Jara, J. L. Guerrero-Naranjo, H. Quiroz-Mercado; Vascular Endothelial Growth Factor Levels in Aqueous, Vitreous, Blood and Subretinal Fluid in Patients With Stage V Retinopathy of Prematurity. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3877.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To measure the levels of vascular endothelial growth factor (VEGF) in aqueous, vitreous and subretinal fluid (SRF) in patients with stage V retinopathy of prematurity (ROP).

Methods: : The study was approved by the hospital ethics board and was performed according to the tenets of the declaration of Helsinki. We included patients with history of prematurity (<32 weeks of gestation), low birth weight (<1500gr) and a clinical diagnosis of stage V ROP, who were scheduled for vitrectomy. We took samples of blood, aqueous, vitreous and SRF. The samples were then analysed by ELISA for Human VEGF. We also sampled the aqueous of healthy patients with clinical diagnosis of congenital cataracts as a control group. Statistical analyses were performed using SPSS Software, version 10.1.0 (Inc. SPSS Chicago, IL).

Results: : We included a total of 16 patients. The average gestational age was 29.56±2.80 weeks. The postconceptional age at the moment of the surgery was 20.94±16.62 months. All the patients had less than 1500gr birth weight of which 8 had less than 1250gr. Forty four percent of patients had an active ROP at the moment of surgery. The remaining 56% did not show activity. The average VEGF levels in aqueous samples were 85.03±61.42pg/ml. In the vitreous: 57.21±49.79pg/ml. In SRF: 1600.63±355.14pg/ml and in blood: 148.25±91.11pg/ml. The average aqueous VEGF level in the control group was 14.67±1.56. All the groups had statistically significant difference with the control group (p<0.001).

Conclusions: : The results in this study show a dramatic increase in the levels of VEGF in all intraocular fluids. The treatment of this pathology with and anti-VEGF drug could be justified.

Clinical Trial: : www.clinicaltrials.gov NCT00500396

Keywords: retinopathy of prematurity • vascular endothelial growth factor • vitreous 
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