May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Relationship Between the Roughness of Corneal Epithelium and the Transmission of Light
P. Perez-Merino; C. Martinez-Garcia; S. Mar-Sardaña; A. Perez-Escudero; J. Merayo-Lloves; T. Blanco-Mezquita; E. Garrido-Calvo
Author Affiliations & Notes
  • P. Perez-Merino
    University of Valladolid, Valladolid, Spain
    Instituto de Oftalmobiología Aplicada, IOBA,
  • C. Martinez-Garcia
    University of Valladolid, Valladolid, Spain
    Departamento de Biología Celular,
  • S. Mar-Sardaña
    University of Valladolid, Valladolid, Spain
    Departamento de Física Teórica, Atómica y Óptica,
  • A. Perez-Escudero
    Instituto de Óptica, CSIC, Madrid, Spain
  • J. Merayo-Lloves
    University of Valladolid, Valladolid, Spain
    Instituto de Oftalmobiología Aplicada, IOBA,
  • T. Blanco-Mezquita
    University of Valladolid, Valladolid, Spain
    Instituto de Oftalmobiología Aplicada, IOBA,
  • E. Garrido-Calvo
    University of Valladolid, Valladolid, Spain
    Departamento de Física Teórica, Atómica y Óptica,
  • Footnotes
    Commercial Relationships  P. Perez-Merino, None; C. Martinez-Garcia, None; S. Mar-Sardaña, None; A. Perez-Escudero, None; J. Merayo-Lloves, None; T. Blanco-Mezquita, None; E. Garrido-Calvo, None.
  • Footnotes
    Support  This work was supported by a grant from the Spanish Ministery of Health (Fondo de Investigación Sanitaria FIS 05/2841).
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3910. doi:
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      P. Perez-Merino, C. Martinez-Garcia, S. Mar-Sardaña, A. Perez-Escudero, J. Merayo-Lloves, T. Blanco-Mezquita, E. Garrido-Calvo; Relationship Between the Roughness of Corneal Epithelium and the Transmission of Light. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3910.

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Abstract

Purpose: : To determine the relationship among transmittance, scattering and epithelial surface properties during wound healing after refractive surgery.

Methods: : Hens operated by different techniques (Lasik, Lasek, PRK), and a group of hens with only epithelial corneal removal but not laser excimer application, were permitted to heal for 30 days. Transmittance and scattering were measured by a scatterometer developed by our group. External and internal roughness and thickness of epithelium were assessed by custom routines developed for quantitative analysis of microphotographs of corneal epithelium.

Results: : Data analysis showed that the epithelial thickness has no influence onthe transmittance. However, higher roughness in the epithelium-stroma interface causes a decrease of transmittance and an increase of scattering. Larger differences between internal and external roughness of epithelium contributes to produce a decrease of transmittance and an increase of scattered light.

Conclusions: : Roughness of epithelial surfaces is directly related to the transmittance and light scattering in the cornea.

Keywords: wound healing • refractive surgery • cornea: epithelium 
© 2008, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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