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P. Perez-Merino, C. Martinez-Garcia, S. Mar-Sardaña, A. Perez-Escudero, J. Merayo-Lloves, T. Blanco-Mezquita, E. Garrido-Calvo; Relationship Between the Roughness of Corneal Epithelium and the Transmission of Light. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3910.
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To determine the relationship among transmittance, scattering and epithelial surface properties during wound healing after refractive surgery.
Hens operated by different techniques (Lasik, Lasek, PRK), and a group of hens with only epithelial corneal removal but not laser excimer application, were permitted to heal for 30 days. Transmittance and scattering were measured by a scatterometer developed by our group. External and internal roughness and thickness of epithelium were assessed by custom routines developed for quantitative analysis of microphotographs of corneal epithelium.
Data analysis showed that the epithelial thickness has no influence onthe transmittance. However, higher roughness in the epithelium-stroma interface causes a decrease of transmittance and an increase of scattering. Larger differences between internal and external roughness of epithelium contributes to produce a decrease of transmittance and an increase of scattered light.
Roughness of epithelial surfaces is directly related to the transmittance and light scattering in the cornea.
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