May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Corneal Collagen Cross-Linking With Riboflavin and UVA Improves Transparency in Hen Corneas
Author Affiliations & Notes
  • T. Blanco
    University Of Valladolid, Valladolid, Spain
    IOBA,
  • J. Merayo-Lloves
    University Of Valladolid, Valladolid, Spain
    IOBA,
  • L. Fabiani
    University Of Valladolid, Valladolid, Spain
    IOBA,
  • S. Del Olmo Aguado
    University Of Valladolid, Valladolid, Spain
    IOBA,
  • S. Mar-Sardana
    University Of Valladolid, Valladolid, Spain
    Optics and physics department,
  • E. Garrido-Calvo
    University Of Valladolid, Valladolid, Spain
    Optics and physics department,
  • J. Cezon
    CIMO, Sevilla, Spain
  • J. Cuevas
    Pathology, Hospital Clinico Universitario, Santiago de Compostela, Spain
  • Footnotes
    Commercial Relationships  T. Blanco, None; J. Merayo-Lloves, None; L. Fabiani, None; S. Del Olmo Aguado, None; S. Mar-Sardana, None; E. Garrido-Calvo, None; J. Cezon, None; J. Cuevas, None.
  • Footnotes
    Support  FIS 052841
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3911. doi:https://doi.org/
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    • Get Citation

      T. Blanco, J. Merayo-Lloves, L. Fabiani, S. Del Olmo Aguado, S. Mar-Sardana, E. Garrido-Calvo, J. Cezon, J. Cuevas; Corneal Collagen Cross-Linking With Riboflavin and UVA Improves Transparency in Hen Corneas. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3911. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate the effect of cross-linking with riboflavin and ultraviolet light (C3RUVA) in hen corneas

Methods: : After mechanical deepithelization, right corneas of 50 lohmann classic hens were crosslinked by application of 0,1% riboflavin-20% dextran solution and exposure to UVA light (360-380 nm, 3 mW/cm2) for 30 minutes. The left fellow control eyes were deepithelized.Time of epithelial closure with fluorescein stain and haze by the Fantes method were evaluated. At different time point, eyes were exenterated and corneal direct transmittance was measured with a scatterometer developed by us. Eyes were fixed for histology and transmission electronic microscopy (TEM) analysis.

Results: : There were not differences among groups in the time of epithelial closure (48±12 hours) and in the grade of haze that was not observed.C3RUVA corneas presented better values of direct transmittance than deepithelized corneas and statistical significant differences (p<0,05) were observed at 1 week, 1, 3 and 6 months after surgery.The collagen fiber diameter observed by TEM in the C3RUVA corneas, was significantly increased by 15%, compared with the control fellow eyes (p<0,05). Although there were not differences among groups, in others parameters of light microscopy (cell death, proliferation and differentiation)

Conclusions: : C3RUVA was well tolerated by hen's corneas with a increase in corneal transparency.The improve in the direct light transmittance observed in C3RUVA corneas could be related with the increase in the collagen fiber diameter.

Keywords: cornea: stroma and keratocytes • refractive surgery • radiation damage: light/UV 
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