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C. Audet, S. Proulx, J. Uwamaliya, A. Deschambeault, P. Carrier, I. Brunette, L. Germain; Optimization of the Culture Conditions for Feline Corneal Endothelial Cell Cultures. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3944.
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To evaluate the effect of various culture conditions and growth supplements on feline corneal endothelial cell (FCEC) cultures.
FCEC were seeded on plastic or on gelatin-coated plates and cultured either with serum alone (0, 5, 10, 20%), with serum and epidermal growth factor (EGF; 0.5, 5, 25, 100 ng/ml) with serum and bovine pituitary extract (BPE; 25, 100 µg/ml) or a combination of both. Cell number, size and morphology were assessed on three different cell populations. Cell cultures were also maintained at confluence to observe the post-confluence changes in cell morphology.
Culturing FCEC on gelatin-coated plates greatly helped in the preservation of an endothelial morphology. EGF and BPE induced proliferation above basal level. The combination of gelatin-coating and supplementing the growth medium with 10% serum, EGF and BPE increased cell numbers above those achieved with serum alone while maintaining a normal endothelial morphology.
Improvements have been made for the culture of FCEC. The final selected medium consistently allows the growth of a contact inhibited cell monolayer of small, polygonal-shaped cells.
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