May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Optimization of the Culture Conditions for Feline Corneal Endothelial Cell Cultures
Author Affiliations & Notes
  • C. Audet
    Ophthalmology and Surgery, LOEX/Laval University, Quebec, Quebec, Canada
  • S. Proulx
    Ophthalmology and Surgery, LOEX/Laval University, Quebec, Quebec, Canada
  • J. Uwamaliya
    Ophthalmology and Surgery, LOEX/Laval University, Quebec, Quebec, Canada
  • A. Deschambeault
    Ophthalmology and Surgery, LOEX/Laval University, Quebec, Quebec, Canada
  • P. Carrier
    Ophthalmology and Surgery, LOEX/Laval University, Quebec, Quebec, Canada
  • I. Brunette
    Department of Ophthalmology, Montreal University, Quebec, Quebec, Canada
  • L. Germain
    Ophthalmology and Surgery, LOEX/Laval University, Quebec, Quebec, Canada
  • Footnotes
    Commercial Relationships  C. Audet, None; S. Proulx, None; J. Uwamaliya, None; A. Deschambeault, None; P. Carrier, None; I. Brunette, None; L. Germain, None.
  • Footnotes
    Support  Canadian Institutes of Health Research (CIHR) Grant, Réseau de Recherche en Santé de la Vision from the Fonds de la Recherche en Santé du Québec (FRSQ)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3944. doi:https://doi.org/
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    • Get Citation

      C. Audet, S. Proulx, J. Uwamaliya, A. Deschambeault, P. Carrier, I. Brunette, L. Germain; Optimization of the Culture Conditions for Feline Corneal Endothelial Cell Cultures. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3944. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate the effect of various culture conditions and growth supplements on feline corneal endothelial cell (FCEC) cultures.

Methods: : FCEC were seeded on plastic or on gelatin-coated plates and cultured either with serum alone (0, 5, 10, 20%), with serum and epidermal growth factor (EGF; 0.5, 5, 25, 100 ng/ml) with serum and bovine pituitary extract (BPE; 25, 100 µg/ml) or a combination of both. Cell number, size and morphology were assessed on three different cell populations. Cell cultures were also maintained at confluence to observe the post-confluence changes in cell morphology.

Results: : Culturing FCEC on gelatin-coated plates greatly helped in the preservation of an endothelial morphology. EGF and BPE induced proliferation above basal level. The combination of gelatin-coating and supplementing the growth medium with 10% serum, EGF and BPE increased cell numbers above those achieved with serum alone while maintaining a normal endothelial morphology.

Conclusions: : Improvements have been made for the culture of FCEC. The final selected medium consistently allows the growth of a contact inhibited cell monolayer of small, polygonal-shaped cells.

Keywords: cornea: endothelium • cornea: basic science • growth factors/growth factor receptors 
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