May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
P38 May Be Involved in Transforming Growth Factor-β2-Induced Cellular Migration in Human Corneal Endothelial Cells
Author Affiliations & Notes
  • T. Joko
    Ehime University Graduate School of Medi, Toon, Japan
    Ophthalmology,
  • S. Tokumaru
    Ehime University Graduate School of Medi, Toon, Japan
    Dermatology,
  • A. Shiraishi
    Ehime University Graduate School of Medi, Toon, Japan
    Ophthalmology,
  • Y. Ohashi
    Ehime University Graduate School of Medi, Toon, Japan
    Ophthalmology,
  • Footnotes
    Commercial Relationships  T. Joko, None; S. Tokumaru, None; A. Shiraishi, None; Y. Ohashi, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3949. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      T. Joko, S. Tokumaru, A. Shiraishi, Y. Ohashi; P38 May Be Involved in Transforming Growth Factor-β2-Induced Cellular Migration in Human Corneal Endothelial Cells. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3949. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To determine whether activation of MAPK signaling pathway is involved in Transforming growth factor-β2 (TGF-β2) -induced cellular migration in human corneal endothelial cells (HCECs) .

Methods: : The effect of recombinant TGF-β2 (10ng/ml) on migration of cultured HCECs was determined by Boyden chamber assay. Western blot analysis and Bio-PlexTM suspension array system (BIO-RAD) were used to determine the phosphorylation of Erk1/2, p38, and JNK in cultured HCECs stimulated by TGF-β2 (10ng/ml). The effect of specific p38 MAPK inhibitor SB239063 (10µM) on TGF-β2-induced cellular migration was determined by Boyden chamber assay.

Results: : TGF-β2 stimulated HCECs migration by 1.7-fold compared with control (p<0.001). TGF-β2 induced p38 phosphorylation by 2-fold, but not Erk1/2 or JNK phosphorylation compared with control at 60 min after stimulation. SB 239063 inhibited TGF-β2-induced cellular migration in HCECs.

Conclusions: : Activation of p38 may be involved in TGF-β2-induced cellular migration in HCECs.

Keywords: cornea: endothelium • signal transduction • wound healing 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×