May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Comparison of Cellular Redox State of Endothelial Cells From Peripheral and Central Cornea
Author Affiliations & Notes
  • Y. Shin
    Ophthalmology, Wilmer Eye Inst Johns Hopkins Hospital, Baltimore, Maryland
  • D. Na
    Ophthalmology, Wilmer Eye Inst Johns Hopkins Hospital, Baltimore, Maryland
  • T. Hongyok
    Ophthalmology, Wilmer Eye Inst Johns Hopkins Hospital, Baltimore, Maryland
  • M. Cano
    Ophthalmology, Wilmer Eye Inst Johns Hopkins Hospital, Baltimore, Maryland
  • R. Chuck
    Ophthalmology, Wilmer Eye Inst Johns Hopkins Hospital, Baltimore, Maryland
  • Footnotes
    Commercial Relationships  Y. Shin, None; D. Na, None; T. Hongyok, None; M. Cano, None; R. Chuck, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3950. doi:https://doi.org/
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    • Get Citation

      Y. Shin, D. Na, T. Hongyok, M. Cano, R. Chuck; Comparison of Cellular Redox State of Endothelial Cells From Peripheral and Central Cornea. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3950. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate and compare the cellular redox state of cultured human endothelial cells from peripheral and central cornea.

Methods: : Corneal endothelial cells from fresh banked human donor tissue not suitable for transplantation were harvested from central and peripheral cornea and cultured on collagen I or poly-D-lysine. Cellular autofluorescence images were obtained using a Zeiss inverted microscope (Thornwood, NY, Axiovert 200M) on day 2. The redox fluorometric ratio (inversely proportional to metabolic rate) was calculated as the net value of fluorescence from the DAPI channel divided by the net value of fluorescence from FITC channel after subtraction of backgrounds.

Results: : The cellular redox ratio was 2.14 ± 0.48 in central and 1.33 ± 0.19 in peripheral cells in poly-D-lysine coating plates and 1.76 ± 0.09 in central and 1.67 ± 0.28 in peripheral cells in collagen coating plates. The cells from central cornea exhibited significantly higher redox ratios than cells from the periphery in poly-D-lysine coating dishes (p=0.010), but were not significantly different in collagen coating dishes (p=0.507).

Keywords: cornea: endothelium • mitochondria • cornea: basic science 
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