May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
The Effect of TGF-β Signal Control on Mouse Corneal Endothelial Cell Proliferation
Author Affiliations & Notes
  • K. Terai
    Ophthalmology, Meiji Univ of Oriental Medicine, Nantan-city, Japan
    Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • J. Yamada
    Ophthalmology, Meiji Univ of Oriental Medicine, Nantan-city, Japan
    Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • S. Kinoshita
    Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • Footnotes
    Commercial Relationships  K. Terai, None; J. Yamada, None; S. Kinoshita, None.
  • Footnotes
    Support  Grand-in-Aid for Scientific Research from the Japanese Ministry of Education, Science and Culture
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3961. doi:
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    • Get Citation

      K. Terai, J. Yamada, S. Kinoshita; The Effect of TGF-β Signal Control on Mouse Corneal Endothelial Cell Proliferation. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3961.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To confirm the effect of TGF-β signal control on damaged mouse corneal endothelial cell proliferation.

Methods: : Eight-week-old C57/BL6 mice were anesthetized and their corneal endothelial cells were damaged by intracameral injection of 0.05% benzalkonium chloride for 15 seconds. In addition, in the transforming growth factor-beta (TGF-β) signal inhibition group, mice were treated by intracameral injection of 10 ug/mL TGF-β receptor-1 kinase inhibitor (CALBIOCHEM; EMD Chemicals, Inc., San Diego, CA). At various time-points after treatment, pictures of anterior segments of the eyes of the mice in each group were taken. Mice were sacrificed 2 hours after intraperitoneal injection of Bromodeoxyuridine (BrdU). Whole eyeballs were fixed by paraform aldehyde and embedded in paraffin, and then thin-sliced sections were made. Those sections were used for Hemaoxylin-Eosin staining or immunostaining for various TGF-β signal mediators. Corneal buttons were stained by alizarin red or stained immunohistochemically by using anti-BrdU antibody.

Results: : Mice corneal endothelial cells disappeared almost entirely by benzalkonium chloride injection. Corneal endothelial cells appeared from 1 or 2 days after benzalkonium chloride injection, and the cell proliferation was up-regulated in the TGF-β signal inhibition group compared to the control group. In observation of the anterior segments of the eyes, corneal transparency was higher in the TGF-β signal inhibition group than in the control group at 7 and 14 days after benzalkonium chloride injection.

Conclusions: : It was confirmed that the TGF-β signal inhibition promoted corneal endothelial cell proliferation in vivo.

Keywords: cornea: endothelium • wound healing • signal transduction 
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