May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Roles of Endogenous TGFbeta and Its Signaling in Migration of Rabbit Corneal Endothelium
Author Affiliations & Notes
  • T. Sumioka
    Ophthalmology, Wakayama Medical University, Wakayama, Japan
  • Y. Okada
    Ophthalmology, Wakayama Medical University, Wakayama, Japan
  • S. Saika
    Ophthalmology, Wakayama Medical University, Wakayama, Japan
  • Footnotes
    Commercial Relationships  T. Sumioka, None; Y. Okada, None; S. Saika, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3963. doi:https://doi.org/
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      T. Sumioka, Y. Okada, S. Saika; Roles of Endogenous TGFbeta and Its Signaling in Migration of Rabbit Corneal Endothelium. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3963. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the roles of endogenous TGF beta-signaling in the migration of rabbit corneal endothelium. Effects of exogenous TGF beta and EGF on it were also studied.

Methods: : An excised rabbit cornea with a scraping partial defect of the endothelial layer was placed in organ-culture. Effects of exogenous EGF or TGF beta1/2 or both on endothelial migration toward the defect was examined. Roles of endogenous TGF beta on endothelial migration were evaluated by using an ALK 5 inhibitor (SB431542). Effects of a JNK inhibitor, a p38MAP kinase inhibitor (SB203580) or a MAP kinase inhibitor (U0126) on such cell migration were also assayed.

Results: : EGF group promoted corneal endothelium spreading compared with control group (p<0.05). Exogenous TGF beta1 and TGF beta2 did not have significant difference compared with control group. TGFbeta1 and TGF beta2 group reversed promotion of corneal endothelium spreading by exogenous EGF (p<0.05). U0126, SB203580 and SB431542 group inhibited corneal endothelium spreading compared with control group (p<0.05). SB203580 group inhibited corneal endothelium spreading more markedly as compared with U0126 group (p<0.05). JNK inhibitor group did not have significant effect on endothelium migration.

Conclusions: : Exogenous TGF beta1/2 counteracts EGF-promotion of migration of corneal endothelial cells, but an endogenous TGF beta signal is essential to endothelium migration. p38 and MAP kinase are both involved in its migration.

Keywords: cornea: endothelium • wound healing • signal transduction 
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