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T. Sumioka, Y. Okada, S. Saika; Roles of Endogenous TGFbeta and Its Signaling in Migration of Rabbit Corneal Endothelium. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3963.
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To investigate the roles of endogenous TGF beta-signaling in the migration of rabbit corneal endothelium. Effects of exogenous TGF beta and EGF on it were also studied.
An excised rabbit cornea with a scraping partial defect of the endothelial layer was placed in organ-culture. Effects of exogenous EGF or TGF beta1/2 or both on endothelial migration toward the defect was examined. Roles of endogenous TGF beta on endothelial migration were evaluated by using an ALK 5 inhibitor (SB431542). Effects of a JNK inhibitor, a p38MAP kinase inhibitor (SB203580) or a MAP kinase inhibitor (U0126) on such cell migration were also assayed.
EGF group promoted corneal endothelium spreading compared with control group (p<0.05). Exogenous TGF beta1 and TGF beta2 did not have significant difference compared with control group. TGFbeta1 and TGF beta2 group reversed promotion of corneal endothelium spreading by exogenous EGF (p<0.05). U0126, SB203580 and SB431542 group inhibited corneal endothelium spreading compared with control group (p<0.05). SB203580 group inhibited corneal endothelium spreading more markedly as compared with U0126 group (p<0.05). JNK inhibitor group did not have significant effect on endothelium migration.
Exogenous TGF beta1/2 counteracts EGF-promotion of migration of corneal endothelial cells, but an endogenous TGF beta signal is essential to endothelium migration. p38 and MAP kinase are both involved in its migration.
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