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S.-J. Sheu, C.-H. Chen; Resveratrol Protects Human Retinal Pigment Epithelial Cells Against Ultraviolet Damage via Large-Conductance Calcium-Activated Potassium Channels. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3965.
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This study was undertaken to examine the protection effect of resveratrol on human retinal pigment epithelial (RPE) cell phagocytosis.
Cultured RPE cells were treated with Ultraviolet A irradiation (UV-A, 20 min), meclofenamic acid (30 µM, 20 min), paxilline (100 nM, 20 min), or resveratrol (10 µM, 20 min) respectively. Meclofenamic acid (30 µM, 20 min) or resveratrol (10 µM, 20 min) was given after exposure to H2O2. Pretreatment with meclofenamic acid (30 µM, 20 min), resveratrol (10 uM, 20 min) or paxilline (100 nM, 20 min) before H2O2 exposure also was performed. Fluorescent latex beads then were fed for 4 hours, and phagocytotic function was assessed by flow cytometry.
UVA irradiation inhibit the phagocytic function of human RPE cells. The BKCa channel inhibitor paxilline inhibited RPE phagocytosis, as did UV-A damage. The BKCa channel opener meclofenamic acid reversed the damage caused by UV-A irradiation. Pretreatment with meclofenamic acid also provided protection against damage caused by UV-A. Posttreatment with resveratrol or meclofenamic acid did not offer protection from UV-A.
The dietary antioxidant resveratrol significantly reduced irradiation damage on human RPE phagocytosis. The underlying mechanism might involve the BKCa channel.
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