May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Resveratrol Protects Human Retinal Pigment Epithelial Cells Against Ultraviolet Damage via Large-Conductance Calcium-Activated Potassium Channels
Author Affiliations & Notes
  • S.-J. Sheu
    Department of Ophthalmology, National Yang-Ming University, Taipei, Taiwan
  • C.-H. Chen
    Department of Ophthalmology, Kaohsiung Veterans Gen Hospital, Kaohsiung, Taiwan
  • Footnotes
    Commercial Relationships  S. Sheu, None; C. Chen, None.
  • Footnotes
    Support  NSC 96-2314-B-075B-004;VGHKS 96-076
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3965. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      S.-J. Sheu, C.-H. Chen; Resveratrol Protects Human Retinal Pigment Epithelial Cells Against Ultraviolet Damage via Large-Conductance Calcium-Activated Potassium Channels. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3965.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : This study was undertaken to examine the protection effect of resveratrol on human retinal pigment epithelial (RPE) cell phagocytosis.

Methods: : Cultured RPE cells were treated with Ultraviolet A irradiation (UV-A, 20 min), meclofenamic acid (30 µM, 20 min), paxilline (100 nM, 20 min), or resveratrol (10 µM, 20 min) respectively. Meclofenamic acid (30 µM, 20 min) or resveratrol (10 µM, 20 min) was given after exposure to H2O2. Pretreatment with meclofenamic acid (30 µM, 20 min), resveratrol (10 uM, 20 min) or paxilline (100 nM, 20 min) before H2O2 exposure also was performed. Fluorescent latex beads then were fed for 4 hours, and phagocytotic function was assessed by flow cytometry.

Results: : UVA irradiation inhibit the phagocytic function of human RPE cells. The BKCa channel inhibitor paxilline inhibited RPE phagocytosis, as did UV-A damage. The BKCa channel opener meclofenamic acid reversed the damage caused by UV-A irradiation. Pretreatment with meclofenamic acid also provided protection against damage caused by UV-A. Posttreatment with resveratrol or meclofenamic acid did not offer protection from UV-A.

Conclusions: : The dietary antioxidant resveratrol significantly reduced irradiation damage on human RPE phagocytosis. The underlying mechanism might involve the BKCa channel.

Keywords: phagocytosis and killing • radiation damage: light/UV • retinal pigment epithelium 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×