May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Differential Toxicity of Ultraviolet Light on Human Retinal Pigment Epithelial, Rat Retinal Neurosensory and Human Lens Epithelial Cells in vitro
Author Affiliations & Notes
  • S. Mansoor
    Ophthalmology, Univ of California Irvine, Irvine, California
  • A. Sharma
    Ophthalmology, Univ of California Irvine, Irvine, California
  • A. Jayaprakash Patil
    Ophthalmology, Univ of California Irvine, Irvine, California
  • M. F. Estrago
    Ophthalmology, Univ of California Irvine, Irvine, California
  • G. M. Seigel
    University at Buffalo, The Ross Eye Institute, New York
  • U. P. Andley
    Washington University, School of Medicine, St Louis, Missouri
  • M. C. Kenney
    Ophthalmology, Univ of California Irvine, Irvine, California
  • B. D. Kuppermann
    Ophthalmology, Univ of California Irvine, Irvine, California
  • Footnotes
    Commercial Relationships  S. Mansoor, None; A. Sharma, None; A. Jayaprakash Patil, None; M.F. Estrago, None; G.M. Seigel, None; U.P. Andley, None; M.C. Kenney, None; B.D. Kuppermann, None.
  • Footnotes
    Support  Discovery Eye Foundation, Iris and B. Gerald Cantor Foundation, Research to Prevent Blindness Foundation; Gilbert Foundation.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3975. doi:https://doi.org/
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      S. Mansoor, A. Sharma, A. Jayaprakash Patil, M. F. Estrago, G. M. Seigel, U. P. Andley, M. C. Kenney, B. D. Kuppermann; Differential Toxicity of Ultraviolet Light on Human Retinal Pigment Epithelial, Rat Retinal Neurosensory and Human Lens Epithelial Cells in vitro. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3975. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate the in vitro toxicity of ultraviolet (UV) light on human retinal pigment epithelial (ARPE-19), rat retinal neurosensory (R28), and human lens epithelial (HLE) cells.

Methods: : ARPE-19 (ATCC, Manassas, VA), R28 (courtesy of GM Seigel), and HLE (courtesy of UP Andley) cells were treated with different intensities of UV light (5000, 10000, 40000, 900000 µjoule/cm2) using the UV Stratalinker (Stratagene, La Jolla, CA). Cell viability was measured using the trypan blue dye exclusion assay at 24 hours.

Results: : All intensities of tested UV light (5000, 10000, 40000, 900000 µjoule/cm2) were found to be safe on the ARPE-19 cells. The mean cell viability of ARPE-19 cells after exposure to 5000, 10000, 40000, 900000 µjoule/cm2 of UV light was 94.3%±1.6 (p>0.05), 95.3%±4.1 (p>0.05), 93.3%±2.7 (p>0.05) and 91.7%±0.7 (p>0.05) respectively compared to untreated ARPE-19 control cells (94.2%±2.9). The mean cell viability of R28 cells after exposure to 5000, 10000, 40000, 900000 µjoule/cm2 of UV light was 72.7%±2.1 (p<0.001), 72.2%±2.9 (p<0.001), 60.6%±0.7 (p<0.001) and 22.2%± 3.0 (p<0.001) respectively compared to untreated R28 controls (87.3%± 2.7). The mean cell viability of HLE cells after exposure to 5000, 10000, 40000, 900000 µjoule/cm2 of UV light was 74.6%±2.0 (p<0.001), 71.6%±2.1 (p<0.001), 14.8%±2.0 (p<0.001) and 2.4%±0.9 (p<0.001) respectively compared to untreated HLE control cells (90.2%±1.5).

Conclusions: : In this study, UV light was non-toxic to ARPE-19 cells. Toxicity was observed in both R28 and HLE cells at all the intensities tested. However, HLE cells were found to be more sensitive to UV light than were R28 cells, with more profound loss of cell viability. These findings correlate with the clinical observation that UV light is cataractogenic but less toxic to the retina.

Keywords: radiation damage: light/UV • retina • retinal culture 
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