May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Omega-3 Fatty Acids Modulate Angiogenic Processes in Retinal Microvascular Endothelial Cells
Author Affiliations & Notes
  • N. Matesanz
    Centre for Vision Science, Queen's University Belfast, Belfast, United Kingdom
  • G. Park
    Centre for Vision Science, Queen's University Belfast, Belfast, United Kingdom
  • A. Devine
    Centre for Vision Science, Queen's University Belfast, Belfast, United Kingdom
  • G. McVeigh
    Centre for Vision Science, Queen's University Belfast, Belfast, United Kingdom
  • T. A. Gardiner
    Centre for Vision Science, Queen's University Belfast, Belfast, United Kingdom
  • D. M. McDonald
    Centre for Vision Science, Queen's University Belfast, Belfast, United Kingdom
  • Footnotes
    Commercial Relationships  N. Matesanz, None; G. Park, None; A. Devine, None; G. McVeigh, None; T.A. Gardiner, None; D.M. McDonald, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3980. doi:
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    • Get Citation

      N. Matesanz, G. Park, A. Devine, G. McVeigh, T. A. Gardiner, D. M. McDonald; Omega-3 Fatty Acids Modulate Angiogenic Processes in Retinal Microvascular Endothelial Cells. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3980.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Dietary supplementation with omega-3 polyunsaturated fatty acids were recently shown to decrease vaso-obliteration and pathological retinal neovascularisation in an animal model of oxygen induced retinopathy. The mechanism for this beneficial effect was demonstrated to be mediated, in part, by a reduction in the expression of the pro-inflammatory cytokine TNFα. Omega-3 fatty acids however, are likely to modulate multiple signalling pathways and the aim of this study was to investigate the direct effects of omega-3 fatty acid supplementation on angiogenic processes in isolated endothelial cells

Methods: : Primary cultures of retinal microvascular endothelial cells (RMECs) were treated with the omega-3 PUFAs, docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) for 24-48h and their incorporation into cellular membranes verified by gas-liquid chromatography. Migration was determined by scratch-wound assay and proliferation by the incorporation of BrdU. Vascular tube formation was determined using a 3-dimensional model of angiogenesis described previously.

Results: : Preloading with DHA and EPA for 24-48h significantly inhibited the ability of retinal endothelial cells to migrate in a scratch-wound assay (30% and 44% respectively (p<0.05). Proliferation was similarly impaired; BrdU incorporation into DHA and EPA treated cells was inhibited by 65% (p<0.001) and 35% (p<0.01)) respectively. In addition, we also observed a significant reduction in vascular tube formation upon treatment with DHA and EPA.

Conclusions: : Omega-3 fatty acids significantly inhibited the migration, proliferation and tube forming ability of retinal endothelial cells. These results demonstrate that in addition to modulating the pro-inflammatory milieu in the hypoxic retina, omega-3 fatty acids also have direct effects on angiogenic signalling pathways in isolated endothelial cells.

Keywords: retinal neovascularization • vascular endothelial growth factor • nitric oxide 
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