May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Trans-Chalcone Prevents VEGF Expression and Retinal Neovascularization in the Ischemic Retina
Author Affiliations & Notes
  • F. Lamoke
    Department of Ophthalmology, University of South Carolina, Columbia, South Carolina
  • M. Labazi
    Vascular Biology Center, Medical College of Georgia, Augusta, Georgia
  • D. Marcus
    Department of Ophthalmology, University of South Carolina, Columbia, South Carolina
  • M. Bartoli
    Department of Ophthalmology, University of South Carolina, Columbia, South Carolina
  • Footnotes
    Commercial Relationships  F. Lamoke, None; M. Labazi, None; D. Marcus, None; M. Bartoli, None.
  • Footnotes
    Support  JDRF, Centenary Plan USC
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3984. doi:https://doi.org/
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    • Get Citation

      F. Lamoke, M. Labazi, D. Marcus, M. Bartoli; Trans-Chalcone Prevents VEGF Expression and Retinal Neovascularization in the Ischemic Retina. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3984. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Retinal neovascularization (RNV) is an important pathological event and a major cause of blindness. Vascular inflammation and oxidative stress have been shown to play a role in the induction and progression of RNV. We characterized the effects of the flavonoid trans-chalcone in preventing RNV in a model of ischemic retinopathy. Trans-chalcone-derived flavonoids have been previously shown to be negative modulators of inflammatory responses as well as tumor angiogenesis.

Methods: : Ischemic retinopathy was induced in neonatal mice following the protocol of the mouse model of oxygen-induced retinopathy (Smith, LEH. et al. 1994). Trans-chalcone was administered intra-peritoneum at the dose of 25mg/Kg/day. Vascular density was assessed by morphometric analysis of flat mounted retinas stained with Texas red-Isolectin B4. Western blotting and immnuno-histochemical analyses were conducted to determine the levels of VEGF, ICAM-1 and the transcriptional activators STAT3 (tyrosine-phosphorylated) and NF-kB (phosphorylated p65).

Results: : Treatment with trans-chalcone significantly inhibited RNV in the ischemic retina, as shown by decreased number of neovascular tufts. Trans-chalcone also blocked ischemia-induced VEGF and ICAM-1 expression and this effect correlated with trans-chalcone-mediated inhibition of activated STAT3 and NF-kB.

Conclusions: : Our results show that trans-chalcone effectively prevents RNV in the murine retina thus suggesting that chalcone-derived flavonoids may be beneficial in preventing pathological neovascularization in the ischemic retina.

Keywords: antioxidants • inflammation • ischemia 
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