May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Differential Expression of Proteins in the Retina of Parkinsonian Monkeys: A Proteomics Approach
Author Affiliations & Notes
  • J. Esteve
    Universidad de Alicante, Alicante, Spain
    Dpto. Fisiología, Genética y Microbiología,
  • L. Campello
    Universidad de Alicante, Alicante, Spain
    Dpto. Fisiología, Genética y Microbiología,
  • R. Bru-Martínez
    Universidad de Alicante, Alicante, Spain
    Dpto. Agroquímica y Bioquímica,
  • M.-T. Herrero
    Dpto. Anatomía Humana y Psicobiología, Universidad de Murcia, Murcia, Spain
  • N. Cuenca
    Universidad de Alicante, Alicante, Spain
    Dpto. Fisiología, Genética y Microbiología,
  • J. Martín-Nieto
    Universidad de Alicante, Alicante, Spain
    Dpto. Fisiología, Genética y Microbiología,
  • Footnotes
    Commercial Relationships  J. Esteve, None; L. Campello, None; R. Bru-Martínez, None; M. Herrero, None; N. Cuenca, None; J. Martín-Nieto, None.
  • Footnotes
    Support  MEC BFU2006-00957/BFI, ONCE and Fundaluce (to N.C.); Generalitat Valenciana GV06/197 (to J.M–N).
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 3991. doi:https://doi.org/
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      J. Esteve, L. Campello, R. Bru-Martínez, M.-T. Herrero, N. Cuenca, J. Martín-Nieto; Differential Expression of Proteins in the Retina of Parkinsonian Monkeys: A Proteomics Approach. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3991. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Systemic injection of MPTP to monkeys elicits the appearance of a parkinsonian syndrome including a set of morphological and functional impairments in the retina. The etiology of idiopathic Parkinson’s disease has been associated with intracellular neuronal oxidative damage. Here we have undertaken a proteomics approach in order to identify proteins differentially expressed in the retina of MPTP-treated, parkinsonian monkeys.

Methods: : Monkeys (Macaca fascicularis) were treated for 2-3 years with MPTP (0.3 mg/kg, i.v.) and then left untreated for 1 additional year. Proteins were solubilized from the neural retinas of control and MPTP-treated animals, labeled with different fluorophores and run pairwise on DIGE gels. Polypeptides showing statistically-significant differences in their expression levels were excised from preparative 2D gels, trypsinized and subjected to MALDI-TOF mass spectrometry analysis. Protein database interrogation was performed by using the MASCOT search engine.

Results: : Out of over 700 protein spots resolved on 2D DIGE gels, ca. 30 polypeptides were detected to show a significant underexpression in the parkinsonian monkey retina, whereas one polypeptide was found overexpressed. Their peptide mass fingerprints obtained by MALDI-TOF were used to search the NCBInr database. A number of the identified differentially-expressed polypeptides corresponded to proteins involved in NO toxicity and cellular energy metabolism.

Conclusions: : We have identified in the MPTP monkey model of idiopathic Parkinson’s disease a set of retinal proteins showing abnormal expression levels in comparison with healthy subjects, in most cases being downregulated. A number of them could be related to an impairment in energy metabolism and oxidative damage, in keeping with one of the prevalent molecular mechanisms underlying the etiology of parkinsonism.

Keywords: retina • proteomics • degenerations/dystrophies 
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