May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Design of a Compact Table-Top Retinal Imaging System for the Mouse
Author Affiliations & Notes
  • O. P. Kocaoglu
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
    Biomedical Optics Laboratory, Department of Biomedical Engineering, University of Miami College of Engineering, Miami, Florida
  • S. R. Uhlhorn
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
  • E. Hernandez
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
  • V. Porciatti
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
  • J.-M. Parel
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
    Biomedical Optics Laboratory, Department of Biomedical Engineering, University of Miami College of Engineering, Miami, Florida
  • F. Manns
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
    Biomedical Optics Laboratory, Department of Biomedical Engineering, University of Miami College of Engineering, Miami, Florida
  • Footnotes
    Commercial Relationships  O.P. Kocaoglu, None; S.R. Uhlhorn, None; E. Hernandez, None; V. Porciatti, None; J. Parel, None; F. Manns, None.
  • Footnotes
    Support  NIH center grant P30-EY014801; Research to Prevent Blindness; The Florida Lions Eye Bank; The Henri and Flore Lesieur Foundation.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4015. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      O. P. Kocaoglu, S. R. Uhlhorn, E. Hernandez, V. Porciatti, J.-M. Parel, F. Manns; Design of a Compact Table-Top Retinal Imaging System for the Mouse. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4015. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To develop a compact table-top imaging system for simultaneous fundus and Optical Coherence Tomography (OCT) imaging of the mouse retina.

Methods: : A compact mouse retinal imaging system providing fundus and OCT images was designed. The mouse fundus imaging system used a digital CCD camera (XCD-SX910CR, Sony, Park Ridge, NJ) with a lens objective and 90 D auxiliary lens (Volk, Mentor, OH). The retina was illuminated with one bright white light-emitting diode (LED) (LED1, Dessau, Germany). The OCT images were acquired with a time-domain system. The OCT beam was coupled on to the optical axis of the fundus camera with a hot mirror and delivered to the mouse eye through the auxiliary Volk lens. The fundus and OCT images are displayed in real-time on a computer monitor. A 6-axis positioning system was mounted in front of the auxiliary lens to align the mouse eye with the optical axis. Fundus and OCT images of C57Bl6 anesthetized mice were acquired simultaneously. The mouse body temperature was controlled during the experiment. To avoid dehydration, one drop of Balanced Salt Solution is applied to the corneal surface at the beginning of the experiment.

Results: : Eight eyes of 4 C57Bl6 mice were successfully imaged. The optic nerve head, vasculature, and nerve fiber layer could be visualized on the fundus images. The system allowed acquisition of OCT images in regions of interest by controlling the position of the OCT scan in real-time with the guidance of the fundus image.

Conclusions: : A compact table-top mouse retinal imaging system suitable for rapid examination of the mouse retina was developed and successfully tested on C57Bl6 mice.

Keywords: retina • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • laser 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×