May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Histomorphological Study and Immunohistochemistry of the Human Ciliary Margin in Retinal Detachment (RD) and Proliferative Vitreoretinopathy (PVR)
Author Affiliations & Notes
  • C. R. Boscher
    Ophthalmology, Centre OPH Etoile, Paris, France
    Ophthalmology, Hopital Américain de Paris, Paris, France
  • Y. Ducournau
    Service d'Anatomie et Cytologie Pathologiques, CHU G et R Laennec, Nantes, France
  • D. Ducournau
    Ophthalmology, Clinique Sourdille, Nantes, France
  • C. Guillaubey
    Anatomie Pathologique, Institut du Forez, Saint Etienne, France
  • J.-F. Mosnier
    Service d'Anatomie et Cytologie Pathologiques, CHU G et R Laennec, Nantes, France
  • Footnotes
    Commercial Relationships  C.R. Boscher, EFFE ENDOSCOPY, C; Y. Ducournau, None; D. Ducournau, None; C. Guillaubey, None; J. Mosnier, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4051. doi:https://doi.org/
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      C. R. Boscher, Y. Ducournau, D. Ducournau, C. Guillaubey, J.-F. Mosnier; Histomorphological Study and Immunohistochemistry of the Human Ciliary Margin in Retinal Detachment (RD) and Proliferative Vitreoretinopathy (PVR). Invest. Ophthalmol. Vis. Sci. 2008;49(13):4051. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : PVR in RD is thought to originate from the Retinal Pigmentary Epithelium (RPE) . Due to poor intraoperative access with conventional vitrectomy, little attention has been given to the Ciliary Epithelium (CE), which is likely the localisation of retinal progenitor cells.

Methods: : Three paraffin-embedded phtisic eviscerated human eyes with RD complicated by anterior and posterior PVR were studied with light microscopy. Normal eye exenterated for orbital tumor served as control. All cases were stained with H & E, and serial sections were immunostained for monoclonal antibody against VEGF, EGFR and the proliferation marker Ki67, as well as for polyclonal antibody glial fibrillary protein (GFAP), and neuron specific enolase (NSE).

Results: : All cases with DR and PVR demonstrated hyperplasia and adenomatous-like proliferation of nonpigmentary pars plana CE; there was a mild and focal proliferation of pigmentary CE. Control retina and CE did not express Ki67; in DR and PVR, a moderate immunostaining for Ki67 was detected, prevalent at the basal layer of the pigmentary CE; there was no Ki67 expression elsewhere in the retina, especially in the RPE. EGFR was strongly expressed at the membrane of some pigmentary CE basal cells, whereas the detached retinal neuroepithelium presented a diffuse cystoplasmic expression.

Keywords: proliferative vitreoretinopathy • retinal detachment • ciliary body 
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