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K. Takeuchi, M. Nakazawa, F. Ishikawa, H. Yamazaki, Y. Miyagawa, T. Metoki, T. Ito; Application of Solid Hyaluronic Acid (Seprafilm®) to the Ophthalmic Surgery. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4145. doi: https://doi.org/.
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We sometimes have to perform re-operation because of unexpected post-operative adhesion after various kinds of ophthalmic surgery.We investigated the effect of solid hyaluronic acid (Seprafilm®) on preventing post-operative wound adhesion by use of animal models.
We first created simple conjunctival incision on the rabbit eyes (n=10). Then Seprafilm was subconjunctivally inserted.As the second experiment, we performed trabeculectomy on the rabbit eyes (n=24), which were divided into 4 groups with or without Seprafilm under and on the scleral flaps, and with or without using 0.04% mitomycin.Histological examination and immunohistochemistry were carried out by using Masson-Trichrome Staining and anti-PCNA antibody, respectively.
Seprafilm significantly prevented subconjunctival scar formation when simply inserted under conjunctival wound. Also, adhision around scleral flap of trabeclectomy was less prevented in eyes trested with Seprafilm than in controls. Immunoreactivity to PCNA disappered after 28 postoperative days in both treated and control groups.
It was thought that use of Seprafilm in the conjunctival wound in eye surgery and also to the trabeculectomy was able to prevent unexpected adhision after eye surgery.Moreover, clinically it was thought that Seprafilm was able to control a function of conjunctival filtrating bleb after trabeculectomy by its charasteristic chemical properties.
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