May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
The Analysis of Hyaluronic Acid as an Antifibrotic Agent and Drug Delivery Platform for 5FU
Author Affiliations & Notes
  • Y. Jiang
    Singapore Eye Research Institute, Singapore, Singapore
  • W. Zheng
    School of Material Engineering, Nanyang Technological University, Singapore, Singapore
  • D. Su
    Singapore National Eye Center, Singapore, Singapore
  • R. Su
    Singapore Eye Research Institute, Singapore, Singapore
  • T. Aung
    Singapore Eye Research Institute, Singapore National Eye Center, Singapore, Singapore
    Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
  • S. Venkatramen
    School of Material Engineering, Nanyang Technological University, Singapore, Singapore
  • T. T. Wong
    Singapore Eye Research Institute, Singapore National Eye Center, Singapore, Singapore
  • Footnotes
    Commercial Relationships  Y. Jiang, None; W. Zheng, None; D. Su, None; R. Su, None; T. Aung, None; S. Venkatramen, None; T.T. Wong, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4148. doi:https://doi.org/
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    • Get Citation

      Y. Jiang, W. Zheng, D. Su, R. Su, T. Aung, S. Venkatramen, T. T. Wong; The Analysis of Hyaluronic Acid as an Antifibrotic Agent and Drug Delivery Platform for 5FU. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4148. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : 5-Fluorouracil injections have been used to prevent bleb failure and scarring. The aim of this study was to investigate the anti-scarring properties of hyaluronic acid (HA) on human Tenon’s myofibroblasts and the rate of 5FU release in a mixture with HA.

Methods: : Human Tenon’s fibroblasts pretreated with TGF-β were cultured with either HA alone or 5-FU/HA (HA concentrations10, 14, 23 mg/ml). Collagen I and alpha smooth muscle actin (ASMA) expression were quantified by RT-PCR. WST-1 assay determined cell viability. 5-FU solution or powder was dispersed in HA and mechanically stirred and then vortex mixed. Quantitation of 5-FU release was carried out by HPLC.

Results: : HA markedly reduced ASMA and collagen I mRNA expression by myofibroblasts compared to controls without HA. Treatment with HA following 5FU (10mg/ml) pretreatment resulted in a greater reduction in ASMA and collagen I expression compared to HA treatment alone, and statistical significance reached with 23mg/ml HA (p<0.05). Cell proliferation and viability was not affected by HA. 5FU solution mixed with HA delayed drug release to 48 hours, with HA of 14mg/ml and 23mg/ml showing a greater delay in drug release than 10mg/ml, (p<0.05). There was no significant difference in rate of drug release between powder and solution form of 5FU although the pH was notably different ie 6.6 and 10 respectively.

Keywords: wound healing • proliferation • drug toxicity/drug effects 
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