Purchase this article with an account.
Y. Jiang, W. Zheng, D. Su, R. Su, T. Aung, S. Venkatramen, T. T. Wong; The Analysis of Hyaluronic Acid as an Antifibrotic Agent and Drug Delivery Platform for 5FU. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4148. doi: https://doi.org/.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
5-Fluorouracil injections have been used to prevent bleb failure and scarring. The aim of this study was to investigate the anti-scarring properties of hyaluronic acid (HA) on human Tenon’s myofibroblasts and the rate of 5FU release in a mixture with HA.
Human Tenon’s fibroblasts pretreated with TGF-β were cultured with either HA alone or 5-FU/HA (HA concentrations10, 14, 23 mg/ml). Collagen I and alpha smooth muscle actin (ASMA) expression were quantified by RT-PCR. WST-1 assay determined cell viability. 5-FU solution or powder was dispersed in HA and mechanically stirred and then vortex mixed. Quantitation of 5-FU release was carried out by HPLC.
HA markedly reduced ASMA and collagen I mRNA expression by myofibroblasts compared to controls without HA. Treatment with HA following 5FU (10mg/ml) pretreatment resulted in a greater reduction in ASMA and collagen I expression compared to HA treatment alone, and statistical significance reached with 23mg/ml HA (p<0.05). Cell proliferation and viability was not affected by HA. 5FU solution mixed with HA delayed drug release to 48 hours, with HA of 14mg/ml and 23mg/ml showing a greater delay in drug release than 10mg/ml, (p<0.05). There was no significant difference in rate of drug release between powder and solution form of 5FU although the pH was notably different ie 6.6 and 10 respectively.
This PDF is available to Subscribers Only