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Y.-D. Kim, K. Cho, M. Park, J.-I. Moon; Effect of Cyclosporin A on Wound Healing After Glaucoma Filtering Surgery in Rabbit. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4151. doi: https://doi.org/.
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Subconjunctival fibrosis is the most common cause of failure of glaucoma filtering surgery. The cyclosporin A(CsA) is an immunosuppressant that can suppress inflammation and fibroblast proliferation and modulate wound healing process. And it has fewer side effects than antimetabolite has. This study has been examined the effect of 0.05% CsA and 0.2% CsA as adjuvant therapy after glaucoma filtering surgery.
The posterior lip sclerotomy was done in 32 eyes of 16 New Zealand white rabbits. Then they were divided into two groups. In one group, 0.05% CsA was instilled into right eyes of rabbits and in the other group, 0.2% CsA was instilled into right eys. Left eyes were remained as control eyes in both groups. Intraocular pressure was measured at 1, 3, 5 days and 1, 2, 4, 8 weeks after the surgery. Hematoxyline & Eosine(H&E) staining and anti-bromodeoxyuridine(BrdU) immunocytochemical staining were also done at 1, 2, 4, 8 weeks.
There was no statistically difference of intraocular pressure between 0.05% CsA experimental group and control group. H&E staining and anti BrdU staining also did not show any difference between two groups. Intraocular pressures of 1 and 2 weeks after the surgery were statistically lower in 0.2% CsA experimental group than in control group. H&E staining was displayed less inflammatory reaction at 2 weeks of 0.2% CsA group than paired control. Fibroblast proliferation rate was also lower at 1 and 2 weeks of 0.2% CsA group.
0.2% CsA could suppress fibroblast proliferation and maintain lower intraocular pressure. This suggests that low concentration CsA might be used as adjuvant therapy with the glaucoma filtering surgery successfully.
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