May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Feasibility Study of Full-Field Optical Coherence Tomography System for an Application of Sub-Cellular Level Retinal Imaging
Author Affiliations & Notes
  • M. Akiba
    TOPCON Advanced Biomedical Imaging Lab, Topcon Medical Systems, Paramus, New Jersey
  • Z. Wang
    TOPCON Advanced Biomedical Imaging Lab, Topcon Medical Systems, Paramus, New Jersey
  • C. Reisman
    TOPCON Advanced Biomedical Imaging Lab, Topcon Medical Systems, Paramus, New Jersey
  • Y. Fukuma
    TOPCON Advanced Biomedical Imaging Lab, Topcon Medical Systems, Paramus, New Jersey
  • M. Hangai
    Ophthalmology and Vision Science, Kyoto University Graduate School of Medicine, Kyoto, Japan
  • N. Yoshimura
    Ophthalmology and Vision Science, Kyoto University Graduate School of Medicine, Kyoto, Japan
  • K. Chan
    TOPCON Advanced Biomedical Imaging Lab, Topcon Medical Systems, Paramus, New Jersey
  • Footnotes
    Commercial Relationships  M. Akiba, TOPCON, E; Z. Wang, TOPCON, E; C. Reisman, TOPCON, E; Y. Fukuma, TOPCON, E; M. Hangai, None; N. Yoshimura, None; K. Chan, TOPCON, E.
  • Footnotes
    Support  NEDO, Japan
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4206. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      M. Akiba, Z. Wang, C. Reisman, Y. Fukuma, M. Hangai, N. Yoshimura, K. Chan; Feasibility Study of Full-Field Optical Coherence Tomography System for an Application of Sub-Cellular Level Retinal Imaging. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4206. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To demonstrate the feasibility of a sub-cellular level retinal imaging application using a newly developed full-field optical coherence tomography (FF-OCT) system.

Methods: : To achieve retinal imaging with a FF-OCT system that we have recently developed for video-rate cellular level imaging in biological tissues (M.Akiba and K.P.Chan, J. Biomed. Opt., in press, 2007), water immersion objectives suitable for retinal imaging were newly designed. As a feasibility study, a simple model eye consisting of a curved acrylic plate as a cornea and a convex lens as a crystalline lens was manufactured to simulate the refraction of a human eye. The model eye was placed in a small container filled with water, and the microscope objective was lowered to the container, where the tissue sample was placed at the fundus plane.

Results: : The transverse resolution was evaluated to be ~4 µm by placing a USAF resolution test target at the fundus plane that was approximately 24 mm apart from the cornea surface of the model eye. The axial resolution was ~3 µm in water as determined by the source bandwidth. The field of view of the present setup was set to 600 µm x 600 µm. Ex vivo porcine retinal tissues were examined. Although retinal nerve fiber layer is depicted as uniformly highly-reflective layer in conventional OCT, it was observed as bundles of highly-reflective fibers in FF-OCT, probably representing retinal nerve fibers.

Conclusions: : The feasibility of retinal imaging using a real-time FF-OCT system incorporated with a newly designed water immersion objective was studied using a model eye. Further modification of the current system may lead to sub-cellular level imaging of human retina in vivo.

Keywords: imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • retina • nerve fiber layer 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×