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M. Akiba, Z. Wang, C. Reisman, Y. Fukuma, M. Hangai, N. Yoshimura, K. Chan; Feasibility Study of Full-Field Optical Coherence Tomography System for an Application of Sub-Cellular Level Retinal Imaging. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4206.
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© ARVO (1962-2015); The Authors (2016-present)
To demonstrate the feasibility of a sub-cellular level retinal imaging application using a newly developed full-field optical coherence tomography (FF-OCT) system.
To achieve retinal imaging with a FF-OCT system that we have recently developed for video-rate cellular level imaging in biological tissues (M.Akiba and K.P.Chan, J. Biomed. Opt., in press, 2007), water immersion objectives suitable for retinal imaging were newly designed. As a feasibility study, a simple model eye consisting of a curved acrylic plate as a cornea and a convex lens as a crystalline lens was manufactured to simulate the refraction of a human eye. The model eye was placed in a small container filled with water, and the microscope objective was lowered to the container, where the tissue sample was placed at the fundus plane.
The transverse resolution was evaluated to be ~4 µm by placing a USAF resolution test target at the fundus plane that was approximately 24 mm apart from the cornea surface of the model eye. The axial resolution was ~3 µm in water as determined by the source bandwidth. The field of view of the present setup was set to 600 µm x 600 µm. Ex vivo porcine retinal tissues were examined. Although retinal nerve fiber layer is depicted as uniformly highly-reflective layer in conventional OCT, it was observed as bundles of highly-reflective fibers in FF-OCT, probably representing retinal nerve fibers.
The feasibility of retinal imaging using a real-time FF-OCT system incorporated with a newly designed water immersion objective was studied using a model eye. Further modification of the current system may lead to sub-cellular level imaging of human retina in vivo.
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