Purchase this article with an account.
S. S. Saunders, D. M. Gattey, W. D. Mathers; Observation of Ophthalmic Viscosurgical Device Properties Using Confocal Microscopy. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4224. doi: https://doi.org/.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To demonstrate a novel approach to imaging the location, distribution and behavioral properties of different classes of Ophthalmic Viscosurgical Devices using confocal microscopy within a simulated anterior chamber.
We obtained 4 different kinds of OVD’s from various categories based on behavioral properties: 1)Provisc - a cohesive agent, 2)Viscoat - a dispersive agent, 3) Healon5 - a "viscoadaptive" agent, and 4)DiscoVisc - a "viscodispersive" agent. Saline suspended PMMA microspheres, which were 1-10 microns in diameter were mixed with the various OVD’s in a ratio of 0.1:5.0ml and observed by light microscopy to ensure uniform distribution. We used a metallic artificial anterior chamber and contact lens to artificially simulate a human anterior chamber and imaged the location and distribution of the various OVD's both before and after a 5.0ml saline washout.
To date, we have found that we can mix the above mentioned microspheres with the various OVD’s and obtain samples that have a uniform distribution of the microspheres within the OVD. We found that PMMA microspheres ranging in size from 1-10 microns were very visible using confocal microscopy, and indeed when mixed in an OVD, allowed us to visualize OVD distribution in both glass slide preparations and in an artificially created anterior chamber. Provisc (a "cohesive" agent) demonstrated a uniform distribution prior to washout and the largest decrease in concentration after washout. Viscoat (a "dispersive" agent) and Healon5 (a "viscoadaptive" agent) showed similar distributions and decreases in concentrations. DiscoVisc (a "viscodispersive" agent) showed a uniform distribution prior to washout and a unique clumping pattern after saline washout.
We report a novel method of examining the location and distribution of Ophthalmic Viscosurgical Devices in an artificial anterior chamber using PMMA microspheres and confocal microscopy. In addition, we were able to observe patterns of OVD retention following simulated anterior chamber washout. Through further development of this imaging technique, we hope to investigate the retention properties of different OVDs in the human eye, in particular, their location and distribution both before and after intraocular procedures.
This PDF is available to Subscribers Only