May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Three-Dimensional Optical Coherence Tomography Imaging of Retinal Sheet Implants in Live Rats
Author Affiliations & Notes
  • M. J. Seiler
    UC Irvine, Irvine, California
    Reeve-Irvine Res Ctr., Sue and Bill Gross Stem Cell Research Ctr., Dept. Anatomy & Neurobiol.,
  • R. Aramant
    UC Irvine, Irvine, California
    Reeve-Irvine Res Ctr., Sue and Bill Gross Stem Cell Research Ctr., Dept. Anatomy & Neurobiol.,
    Dept. Anatomy & Neurobiol., Univ. Louisville, Louisville, Kentucky
  • B. Rao
    UC Irvine, Irvine, California
    Dept. Biomed. Engineering, Beckman Laser Inst.,
    Dept. Electrical Engineering & Computer Science,
  • L. Yu
    UC Irvine, Irvine, California
    Dept. Biomed. Engineering, Beckman Laser Inst.,
  • Q. Wang
    UC Irvine, Irvine, California
    Dept. Biomed. Engineering, Beckman Laser Inst.,
  • S. Pham
    UC Irvine, Irvine, California
    Dept. Biomed. Engineering, Beckman Laser Inst.,
  • Z. Chen
    UC Irvine, Irvine, California
    Dept. Biomed. Engineering, Beckman Laser Inst.,
  • F. Yan
    UC Irvine, Irvine, California
    Reeve-Irvine Res Ctr., Sue and Bill Gross Stem Cell Research Ctr., Dept. Anatomy & Neurobiol.,
  • H. K. Keirstead
    UC Irvine, Irvine, California
    Reeve-Irvine Res Ctr., Sue and Bill Gross Stem Cell Research Ctr., Dept. Anatomy & Neurobiol.,
  • Footnotes
    Commercial Relationships  M.J. Seiler, Ocular Transplantation LLC, P; R. Aramant, Ocular Transplantation LLC, P; Ocular Transplantation LLC, E; B. Rao, None; L. Yu, None; Q. Wang, None; S. Pham, None; Z. Chen, None; F. Yan, None; H.K. Keirstead, None.
  • Footnotes
    Support  Lincy Foundation, NIH (EB-00293, NCI-91717, RR-01192), Air Force Office of Science Research (FA9550-04-1-0101), Institutional support from BLI and Beckman Medical Clinic.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4287. doi:https://doi.org/
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    • Get Citation

      M. J. Seiler, R. Aramant, B. Rao, L. Yu, Q. Wang, S. Pham, Z. Chen, F. Yan, H. K. Keirstead; Three-Dimensional Optical Coherence Tomography Imaging of Retinal Sheet Implants in Live Rats. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4287. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To obtain three-dimensional images from retinal transplants in live animals and evaluate the placement and structural quality of the transplants.

Methods: : Donor retinal sheets were isolated from E19 fetuses of transgenic rats expressing human alkaline phosphatase (hPAP) as a marker, and transplanted to the subretinal space of 24 - 56 d old S334ter-3 rat recipients with fast retinal degeneration. Forty-one rats were imaged 6 - 67 d after transplant surgery, at the age of 36 to 107 d, using a Fourier domain optical coherence tomography (FDOCT) system, with an axial resolution of 3.5 micron. The CCD A-line integration time was set at 200 µs for better visualization of degenerated retina. After targeting the transplant area, 198 consecutive slices were scanned. From these scans, 3D-projection images and movies of the retinal transplant area were computed.

Results: : Transplants could easily be identified. OCT scans also showed whether the transplants had been correctly placed into the subretinal space, and whether there had been damage to the RPE or choroid during implantation. OCT indicated the laminar structure of the implants. Three-dimensional projections showed the transplant position in the retina in relation to the optic disc. Transplants were identified histologically by hPAP staining.

Conclusions: : Optical Coherence Tomography is an excellent tool to image retinal layers in a live rat. This is important to assess the transplant success early after surgery. This procedure helps to evaluate the placement and layering of the implants in the living eye.

Keywords: imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • imaging/image analysis: non-clinical • transplantation 
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