May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Cytoprotective Effects of a Blue-Light-Absorbing IOL on Human RPE by Reduced Phototoxic Decrease of Bcl-2
Author Affiliations & Notes
  • M. Kernt
    Ophtalmology, Augenklinik der Universitat Muenchen, Gruenwald, Germany
  • U. Welge-Lussen
    Ophtalmology, Augenklinik der Universitat Muenchen, Gruenwald, Germany
  • R. Liegl
    Ophtalmology, Augenklinik der Universitat Muenchen, Gruenwald, Germany
  • A. S. Neubauer
    Ophtalmology, Augenklinik der Universitat Muenchen, Gruenwald, Germany
  • A. Kampik
    Ophtalmology, Augenklinik der Universitat Muenchen, Gruenwald, Germany
  • Footnotes
    Commercial Relationships  M. Kernt, None; U. Welge-Lussen, None; R. Liegl, None; A.S. Neubauer, None; A. Kampik, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4290. doi:https://doi.org/
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      M. Kernt, U. Welge-Lussen, R. Liegl, A. S. Neubauer, A. Kampik; Cytoprotective Effects of a Blue-Light-Absorbing IOL on Human RPE by Reduced Phototoxic Decrease of Bcl-2. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4290. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Methods: : Primary human RPE cells were exposed to white light and either a SN60AT or SA60AT IOL was placed in the light beam. After 10 to 60 minutes of irradiation, the viability of the cells was determined by a colorimetric test (MTT), and expression of VEGF-A and Bcl-2 and their mRNA were determined by RT-PCR and Western-Blot analysis.

Results: : Without any IOL, white-light exposure decreased cell viability compared to the nonirradiated control in a time-of-irradiation-dependent manner. Light-induced cell death was significantly reduced by both, the SN60AT and SA60AT IOL. The blue-light filtering of the SN60AT further significantly attenuated light-induced cell damage as compared to the SA60AT IOL. RT-PCR and Western-Blot analysis yielded a significant, time-of-irradiation-dependent decrease of Bcl-2 and an increase of VEGF-A. In the presence of an IOL, Bcl-2 levels decreased and VEGF-A levels increased significant less. In addition, this decrease of Bcl-2 and increase of VEGF-A was significantly less for the SN60AT IOL compared to the SA60AT IOL.

Keywords: intraocular lens • age-related macular degeneration • apoptosis/cell death 
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