Abstract
Methods: :
Primary human RPE cells were exposed to white light and either a SN60AT or SA60AT IOL was placed in the light beam. After 10 to 60 minutes of irradiation, the viability of the cells was determined by a colorimetric test (MTT), and expression of VEGF-A and Bcl-2 and their mRNA were determined by RT-PCR and Western-Blot analysis.
Results: :
Without any IOL, white-light exposure decreased cell viability compared to the nonirradiated control in a time-of-irradiation-dependent manner. Light-induced cell death was significantly reduced by both, the SN60AT and SA60AT IOL. The blue-light filtering of the SN60AT further significantly attenuated light-induced cell damage as compared to the SA60AT IOL. RT-PCR and Western-Blot analysis yielded a significant, time-of-irradiation-dependent decrease of Bcl-2 and an increase of VEGF-A. In the presence of an IOL, Bcl-2 levels decreased and VEGF-A levels increased significant less. In addition, this decrease of Bcl-2 and increase of VEGF-A was significantly less for the SN60AT IOL compared to the SA60AT IOL.
Keywords: intraocular lens • age-related macular degeneration • apoptosis/cell death