May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Co-Factors of Lim Domains (clims/ldb/nli) Control Corneal Epithelial Cell Fate
Author Affiliations & Notes
  • Z. Yu
    Medicine, University of California Irvine, Irvine, California
  • X. Xu, Sr.
    Medicine, University of California Irvine, Irvine, California
  • B. Andersen
    Medicine, University of California Irvine, Irvine, California
  • Footnotes
    Commercial Relationships  Z. Yu, None; X. Xu, None; B. Andersen, None.
  • Footnotes
    Support  EY016425
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4309. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Z. Yu, X. Xu, Sr., B. Andersen; Co-Factors of Lim Domains (clims/ldb/nli) Control Corneal Epithelial Cell Fate. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4309. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : The corneal epithelium is maintained by corneal epithelial stem cells that differentiate into a stratified and nonkeratinized epithelium, which is essential for a transparent cornea. We have previously shown that corneal epithelial expression of a dominant-negative Co-factor of LIM domains (DN-Clim) leads to transdifferentiation of corneal epithelial cells to epidermis-like epithelium and blindness. Our purpose is to elucidate the molecular mechanism underlying corneal cell fate maintenance by Clims.

Methods: : We established Keratin 14-DN-Clim transgenic mice, and used immunostaining to investigate key signaling pathways that are involved in corneal epithelial cell fate.

Results: : We found that interfering with binding of Clim co-factors to transcription factors causes corneal epithelial cell switch to epidermis like cells. Furthermore, Pax 6, an important transcriptional factor for corneal cell fate maintenance, is strikingly downregulated in the highly hyperplastic corneal epithelium. We also found that beta-catenin is upregulated in the corneas of keratin14-DN-Clims transgenic mice.

Keywords: cornea: epithelium • cornea: clinical science 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×