May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Distribution of ROCK2 in TNF--Induced Optic Nerve Degeneration
Author Affiliations & Notes
  • Y. Kitaoka
    Ophthalmology, St. Marianna University School of Med, Kawasaki, Japan
  • Y. Kitaoka
    Ophthalmology, St. Marianna University School of Med, Kawasaki, Japan
  • H. Takeda
    Ophthalmology, St. Marianna University School of Med, Kawasaki, Japan
  • H. Fujino
    Ophthalmology, St. Marianna University School of Med, Kawasaki, Japan
  • Y. Hayashi
    Ophthalmology, St. Marianna University School of Med, Kawasaki, Japan
  • S. Ueno
    Ophthalmology, St. Marianna University School of Med, Kawasaki, Japan
  • Footnotes
    Commercial Relationships  Y. Kitaoka, None; Y. Kitaoka, None; H. Takeda, None; H. Fujino, None; Y. Hayashi, None; S. Ueno, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4357. doi:https://doi.org/
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      Y. Kitaoka, Y. Kitaoka, H. Takeda, H. Fujino, Y. Hayashi, S. Ueno; Distribution of ROCK2 in TNF--Induced Optic Nerve Degeneration. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4357. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Several studies reported that inactivation of the Rho/ROCK signaling pathway promoted central nervous system axon regeneration. The purpose of current study is to examine the distribution of ROCK2 in TNF-α-induced optic nerve degeneration.

Methods: : Rats were euthanatized at 1 day, or 1 or 2 weeks after PBS or TNF-α (10 ng) intravitreal injection. Levels of ROCK2 protein in the optic nerve and retina were evaluated by Western blot analysis. Localization and distribution of ROCK2 in optic nerve cross section as well as transverse section were determined by immunohistochemistry.

Results: : Western blot analysis showed a significant increase in ROCK2 protein levels in the optic nerve at 1 day after TNF-α injection. Immunohistochemical double labeling showed substantial co-localization of ROCK2 and vimentin in the optic nerve as well as retina. There was a decrease in immunoreactivity of neurofilament in the optic nerve 2 weeks after TNF-α injection. Abundant immunoreactivity of ROCK2 was observed in post-laminar portion and this area had an increased immunoreactivity of vimentin after TNF-α injection.

Conclusions: : Increase of ROCK2 in the optic nerve may be involved in increased vimentin activity around axons in TNF-α-induced optic nerve degeneration.

Keywords: neuro-ophthalmology: optic nerve • optic nerve • glia 
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