May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Neural Crest-Derived Tissue Plasminogen Activator Within the Mammalian Eye:Anatomic Distribution Revealed by a New Transgenic Marker
Author Affiliations & Notes
  • Z. Hao
    University of Connecticut Health Center, Farmington, Connecticut
  • C. Guo
    Howard Hughes Medical Institute, Ashburn, Virginia
  • S. Dufour
    Institute Curie, Paris, France
  • X. Jiang
    University of Connecticut Health Center, Farmington, Connecticut
  • R. Cone
    University of Connecticut Health Center, Farmington, Connecticut
  • D.-N. Hu
    New York Eye and Ear Institute, New York City, New York
  • N. Charkraborty
    University of Connecticut Health Center, Farmington, Connecticut
  • J. O'Rourke
    University of Connecticut Health Center, Farmington, Connecticut
  • Footnotes
    Commercial Relationships  Z. Hao, None; C. Guo, None; S. Dufour, None; X. Jiang, None; R. Cone, None; D. Hu, None; N. Charkraborty, None; J. O'Rourke, None.
  • Footnotes
    Support  NIH EY 01243
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4368. doi:https://doi.org/
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      Z. Hao, C. Guo, S. Dufour, X. Jiang, R. Cone, D.-N. Hu, N. Charkraborty, J. O'Rourke; Neural Crest-Derived Tissue Plasminogen Activator Within the Mammalian Eye:Anatomic Distribution Revealed by a New Transgenic Marker. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4368. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Long thin sympathetic axons transport tissue plasminogen activator(t-PA) to the eye. There it is released in response to adrenergic stimulations. The t-PA is synthesized and packaged in transport vesicles in superior cervical sympathetic ganglion neuron cell bodies(1) .Plasmin activated by t-PA has long been thought to accelerate the trabecular outflow of aqueous humor. Our purpose here is to map the neural crest origins of cells able to produce t-PA within the eye.

Methods: : A promoter mouse line- whose human t-PA Cre transgene is specifically expressed by sympathetic nerves and all other crest derivatives(2)- was crossed with a Lac Z reporter expressing the enhanced green fluorescent protein(EGFP) transgene. PCR sorted pups showed both t-PA promoter and EGFP expressions confined within crest-derived cells. Cryosections were viewed by confocal and UV microscopy ,and immunostained for t-PA antigen.Cultured human uveal melanocytes were stimlated with phenylephrine to confirm a t-PA enzyme release; and immunostained to reveal cell membrane alpha 1 adrenergic receptors.

Results: : Bright discreet promoter expressions ,to be shown ,appeared in fine (0.5-2.0 micron wide) crest- derived uveal and corneal nerve filaments;corneal and trabecular endothelium; uveal melanocytes;and unexpectedly in retinal ganglion,bipolar and, photoreceptor cells(see figure below), the optic nerve and chiasm. Cultured melanocyte t-PA basal release increased 70-80% following alpha 1 receptor stimulations.

Keywords: anatomy 
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